BACKGROUND:Breast cancer is the most common malignant tumor in women and highly heterogeneous with a variety of different molecular subtypes. The analysis of the individual tumor biology is necessary to develop a specific and individualized treatment plan for every patient. The chick chorioallantoic membrane (CAM) model, a 3D-in-vivo-tumor-model, could potentially provide a methodology that facilitates the gain of additional information regarding the tumor biology as well as the testing of the tumor’s individual sensitivity to different therapies. OBJECTIVE:The objective was to establish the grafting of different breast cancer primaries onto the CAM for tumor profiling and the investigation of different parameters. METHODS:Breast cancer primary tissue of different patients was grafted onto the CAM. Subsequently, 3D volume and perfusion measurements were performed during the engraftment period. Histological analyses of the tumors were carried out after the engraftment period. RESULTS:The grafting of the breast cancer primaries onto the CAM was successful. The tumors remained partially vital and displayed angiogenic development on the CAM. CONCLUSIONS:Breast cancer primary material can be grafted onto the CAM and we observed visible and measurable changes of perfusion over time.

中文翻译：

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在 3D 体内 模型中评估乳腺癌原发肿瘤材料

背景：乳腺癌是女性最常见的恶性肿瘤，具有高度异质性，具有多种不同的分子亚型。对个体肿瘤生物学的分析对于为每位患者制定特定的个体化治疗计划是必要的。鸡绒毛尿囊膜 (CAM) 模型是一种 3D 体内肿瘤模型，可能提供一种方法，有助于获得有关肿瘤生物学的额外信息，以及测试肿瘤个体对不同疗法的敏感性。目的：目的是建立不同乳腺癌原发灶移植到 CAM 上的肿瘤分析和不同参数的研究。方法：将不同患者的乳腺癌原发组织移植到CAM上。随后，在植入期间进行 3D 体积和灌注测量。在移植期之后进行肿瘤的组织学分析。结果：原发乳腺癌移植到CAM上成功。肿瘤保持部分活力，并在 CAM 上显示出血管生成发展。结论：乳腺癌原发材料可以移植到 CAM 上，我们观察到灌注随时间的可见和可测量变化。