β-Caryophyllene (BCP), a bicyclic sesquiterpene, has proved to exhibit antioxidant and anti-inflammatory activities. The present study is carried out to investigate BCP impact on hyperoxaluria-induced kidney dysfunction in male Wistar rats. The animals were categorized into four groups, namely, Group I, control rats; Group II, ethylene glycol (inducer); Group III, inducer + BCP (100 µM/kg bw); Group IV, BCP alone. After the treatment period, the rate of creatinine clearance and the concentration of urea in urine and serum were assessed. Histopathology reports were conducted to study renal and liver tissues, while the reverse transcription-polymerase chain reaction studies were carried out for messenger RNA expression of inflammatory (nuclear factor kappa B) and endoplasmic reticulum (ER) stress (kidney dysfunction molecule-1, monocyte chemoattractant protein-1, glucose binding protein 78, CHOP, activating factor 4, and X-box binding protein-1) markers as well as antioxidant activity for the hyperoxaluric rats. Western blot was performed to investigate the level of protein expression by the treatment group on apoptotic (Bcl-2, Bax, caspase-3, and caspase-9) proteins. The results show BCP to possess a renoprotective effect under hyperoxaluric conditions by decreasing the level of the inflammatory and ER stress markers and restoring the enzymes' antioxidant activities. The histology reports depicted the satisfactory morphology of glomerulus in diseased rats. Furthermore, the results of Western blot suggested that BCP may possess inhibitory action on apoptosis by affecting the mitochondrial-dependent apoptotic pathway. Therefore, BCP can be considered as a potential candidate for the therapy of hyperoxaluric-induced kidney complications.

中文翻译：

---

β-石竹烯可通过调节应激标志物 KIM-1/MCP-1 和 NF-κB 信号通路减轻高草酸尿引起的大鼠肾功能障碍

β-石竹烯 (BCP) 是一种双环倍半萜，已被证明具有抗氧化和抗炎活性。本研究旨在调查 BCP 对雄性 Wistar 大鼠高草酸引起的肾功能障碍的影响。将动物分为四组，即第一组，对照大鼠；第二组，乙二醇（诱导剂）；第 III 组，诱导剂 + BCP (100 µM/kg bw)；第四组，单独的 BCP。治疗期结束后，评估肌酐清除率以及尿液和血清中的尿素浓度。组织病理学报告用于研究肾脏和肝脏组织，而逆转录聚合酶链反应研究则用于炎症（核因子κB）和内质网（ER）应激（肾功能障碍分子-1，单核细胞趋化蛋白 1、葡萄糖结合蛋白 78、CHOP、激活因子 4 和 X-box 结合蛋白 1) 标志物以及高草酸大鼠的抗氧化活性。进行蛋白质印迹以研究治疗组对凋亡蛋白（Bcl-2、Bax、caspase-3 和 caspase-9）的蛋白表达水平。结果表明，BCP 通过降低炎症和 ER 应激标志物的水平并恢复酶的抗氧化活性，在高草酸条件下具有肾脏保护作用。组织学报告描述了患病大鼠肾小球的令人满意的形态。此外，Western blot的结果表明，BCP可能通过影响线粒体依赖性凋亡通路对细胞凋亡具有抑制作用。所以，