Investigation of the Action of Peptides on Lipid Membranes,The Journal of Physical Chemistry B

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Investigation of the Action of Peptides on Lipid Membranes
The Journal of Physical Chemistry B ( IF 2.8 ) Pub Date : 2021-08-31 , DOI: 10.1021/acs.jpcb.1c06388
Aline Cisse _{1,

2} , Arnaud Marquette ₃ , Munkhtuguldur Altangerel _{1,

2} , Judith Peters _{1,

2,

4} , Burkhard Bechinger _{3,

4}

Affiliation

Calorimetric and incoherent neutron scattering methods were employed to investigate the action of magainin 2 and PGLa peptides on the phase behavior and molecular dynamics of lipids mimicking cytoplasmic membranes of Gram-negative bacteria. The impact of the peptides, tested individually and cooperatively by differential scanning calorimetry, presented a broadened peak, sometimes with a second shoulder, depicting the phase transition temperature around 21 °C. Neutron scattering revealed a small but significant variation of the membrane dynamics due to the peptides in both in-plane and out-of-plane directions. Although we did not find a clear hint for synergy in the interplay of the two peptides, the calorimetric and neutron data give compatible results in terms of a decrease of the enthalpy due to the presence of the peptides, which destabilize the membrane. The dynamics in the two directions was differentiated when the individual peptides were added to the membranes, but the impact was smaller when both peptides were added together.

中文翻译：

肽对脂质膜作用的研究

采用量热法和非相干中子散射方法研究 magainin 2 和 PGLa 肽对模拟革兰氏阴性菌细胞质膜的脂质的相行为和分子动力学的作用。肽的影响，通过差示扫描量热法单独和协同测试，呈现出一个加宽的峰，有时带有第二个肩峰，描绘了 21 °C 左右的相变温度。中子散射揭示了由于肽在平面内和平面外方向上的微小但显着的膜动力学变化。尽管我们没有发现两种肽相互作用的协同作用的明确提示，但量热和中子数据在由于肽的存在而导致的焓降低方面给出了相容的结果，使膜不稳定。当将单个肽添加到膜中时，两个方向的动力学是不同的，但是当两种肽一起添加时影响较小。

更新日期：2021-09-16

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