Dispersants with a low environmental impact are necessary for oil spill remediation. Thus, the biosurfactant produced by the bacterium Pseudomonas cepacia CCT6659 using industrial waste and formulated using a method of adding food preservative was evaluated for its toxicity against the vegetable Allium cepa and aquatic species such as the freshwater fish Poecilia vivipara and the bivalve Anomalocardia brasiliana. The biosurfactant was also applied in the removal of hydrophobic contaminant in sand and soils, in sea stones, in the dispersion and in the bioremediation of oil in seawater. The surfactant was considered to be of low toxicity for the bioindicators evaluated. As a petroderivative removal agent, the formulated tensoactive reached 76.55 % removal in soil and 84.50 % in sea stones. The biosurfactant was able to disperse 96.00 % of oil in seawater and promoted an increase in the biodegradation of oil by 70.00 % during a bioremediation process carried out in seawater. Therefore, the formulated biosurfactant presents suitable conditions for application as a dispersing agent in the decontamination of terrestrial and aquatic environments impacted by oil spills in substitution of chemical and toxic compounds.

对环境影响小的分散剂是溢油修复所必需的。因此，评估了由洋葱假单胞菌CCT6659 使用工业废物生产并使用添加食品防腐剂的方法配制的生物表面活性剂对蔬菜洋葱和水生物种（如淡水鱼Poecilia vivipara和双壳类Anomalocardia brasiliana）的毒性. 生物表面活性剂还用于去除沙子和土壤、海石中的疏水性污染物，以及海水中油的分散和生物修复。表面活性剂被认为对所评估的生物指示剂具有低毒性。作为一种石油衍生物去除剂，配制的表面活性剂在土壤中的去除率达到了 76.55%，在海石中的去除率达到了 84.50%。在海水中进行生物修复过程中，生物表面活性剂能够将 96.00% 的油分散到海水中，并促进油的生物降解增加 70.00%。因此，配制的生物表面活性剂具有合适的条件，可用作分散剂以替代化学和有毒化合物，用于净化受石油泄漏影响的陆地和水生环境。