Injectable silk nanofiber hydrogels as stem cell carriers to accelerate wound healing,Journal of Materials Chemistry B

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Injectable silk nanofiber hydrogels as stem cell carriers to accelerate wound healing
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2021-08-26 , DOI: 10.1039/d1tb01320c
Jiadai Li _{1,

2} , Zhaozhao Ding ₁ , Xin Zheng ₃ , Guozhong Lu ₄ , Qiang Lu ₁ , David L Kaplan ₅

Affiliation

Stem cells have potential utility in wound therapy, however the benefits are often limited due to cell injury from shear stress during injection and poor retention at the wound site. Here, shear-thinning silk nanofiber hydrogels were used to load bone marrow derived mesenchymal stem cells (BMSCs) and inject into wound sites to optimize cell retention and accelerate wound healing. The BMSCs in the silk nanofiber hydrogels maintained stemness better than the cells cultured on plates, and the expression of wound healing-related genes was significantly higher in the hydrogels with higher silk concentrations (2 wt%). The silk nanofibers physically prevented migration of BMSCs from the deposition site in the wound bed. In addition to faster wound healing, these BMSC-loaded hydrogels mediated angiogenesis and inflammation and improved collagen deposition and hair follicle regeneration in vivo in rats. Considering that these silk nanofiber hydrogels were successfully used here as carriers for stem cells to accelerate wound healing, further study for skin regeneration may be warranted.

中文翻译：

可注射丝纳米纤维水凝胶作为干细胞载体加速伤口愈合

干细胞在伤口治疗中具有潜在的用途，但由于注射过程中剪切应力造成的细胞损伤以及伤口部位的保留不良，其益处往往受到限制。在这里，剪切稀化的丝纳米纤维水凝胶被用来加载骨髓间充质干细胞（BMSC）并注射到伤口部位，以优化细胞保留并加速伤口愈合。丝纳米纤维水凝胶中的BMSC比在平板上培养的细胞更好地保持干性，并且在丝浓度较高（2wt%）的水凝胶中伤口愈合相关基因的表达显着更高。丝纳米纤维从物理上阻止了骨髓间充质干细胞从伤口床沉积部位的迁移。除了加快伤口愈合之外，这些负载 BMSC 的水凝胶还能介导血管生成和炎症，并改善大鼠体内胶原蛋白沉积和毛囊再生。考虑到这些丝纳米纤维水凝胶已成功用作干细胞载体以加速伤口愈合，因此可能需要进一步研究皮肤再生。

更新日期：2021-09-01

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