Instead of using the lipid II substrate that requires prior labelling with a radioactive isotope or fluorophore to probe the formation of peptidoglycan in bacterial transglycosylation, the released undecaprenyl pyrophosphate (UPP) product is quantitatively measured either using a terpyridine–zinc fluorescence turn-on sensor or simply by the second-order scattering effect of the in situ formed UPP–calcium complex. Both the assay methods are utilized to identify moenomycin A as a potent transglycosylase inhibitor with a consistent IC₅₀ value.

中文翻译：

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通过分子开启传感和二阶散射效应有效测定细菌转糖基化

无需使用需要事先用放射性同位素或荧光团标记的脂质 II 底物来探测细菌转糖基化中肽聚糖的形成，而是使用三联吡啶-锌荧光开启传感器或简单地通过原位形成的 UPP-钙复合物的二阶散射效应。两种测定方法都用于将莫诺霉素 A 鉴定为具有一致 IC ₅₀值的有效转糖基酶抑制剂。