Bacillary dysentery is a common foodborne disease with an exaggerated mortality rate because of Shigella infection. With the increasing severity of Shigella infection, lyase has been considered as the most promising alternative to antimicrobial agents, owing to the emergence of resistant bacteria and the difficulty in disrupting and eliminating bacterial biofilms. In this study, we cloned and characterised HolSSE1 and LysSSE1, holin, and lysozyme from the S. dysenteriae phage SSE1 with extended bacterial host range against common gram-negative and gram-positive bacteria. In addition, the efficacy of HolSSE1 and LysSSE1 in removing bacterial biofilms was observed on polystyrene surfaces. Moreover, synergistic bacteriostasis was observed when they were used together. Alignment and structural model analysis showed that both HolSSE1 and LysSSE1 are T4 phage proteins that have not yet been identified. Therefore, HolSSE1 and LysSSE1 can be promising biocontrol agents for the prevention and treatment of various pathogenic infections.

细菌性痢疾是一种常见的食源性疾病，由于志贺氏菌感染，死亡率很高。随着志贺氏菌感染严重程度的增加，裂解酶被认为是最有希望的抗菌药物替代品，因为耐药菌的出现以及破坏和消除细菌生物膜的困难。在这项研究中，我们克隆并表征了来自痢疾链球菌的 HolSSE1 和 LysSSE1、穴蛋白和溶菌酶噬菌体 SSE1 具有针对常见革兰氏阴性和革兰氏阳性细菌的扩展细菌宿主范围。此外，在聚苯乙烯表面观察到 HolSSE1 和 LysSSE1 在去除细菌生物膜方面的功效。此外，当它们一起使用时，观察到协同抑菌。比对和结构模型分析表明，HolSSE1 和 LysSSE1 都是尚未鉴定的 T4 噬菌体蛋白。因此，HolSSE1 和 LysSSE1 可以成为预防和治疗各种病原体感染的有前途的生物防治剂。