Microbial astaxanthin with strong antioxidant activity is greatly demanded for diverse applications. Extractive disruption in aqueous biphasic system (ABS) integrates the cells disruption and biomolecules recovery processes in one-step operation, allowing the direct recovery of intracellular biomolecules with biphasic system upon released from cells. In this study, astaxanthin was recovered from recombinant Kluyveromyces marxianus yeast cells via extractive disruption using alcohol/salt ABS. Recombinant K. marxianus yeast is engineered to produce high concentration of free form astaxanthin. Highest partition coefficient (K = 90.02 ± 2.25) and yield (Y = 96.80% ± 0.05) of astaxanthin were obtained with ABS composed of 20% (w/w) 1-propanol and 20% (w/w) sodium citrate of pH 5, 0.5% (w/w) yeast cells loading and additional of 1% (w/w) 1-butyl-3-methylimidazolium tetrafluoroborate (Bmim)BF₄ to improve the migration of astaxanthin to alcohol-rich top phase. The incorporation of 2.5 h of ultrasonication to the biphasic system further enhanced the astaxanthin recovery in ABS. The direct recovery of astaxanthin from recombinant K. marxianus cells was demonstrated with the ultrasonication-assisted alcohol/salt ABS which integrates the extraction and concentration of astaxanthin in a single-step operation.

具有强抗氧化活性的微生物虾青素被广泛应用于各种用途。水性双相系统 (ABS) 中的萃取破坏将细胞破坏和生物分子回收过程整合在一步操作中，允许从细胞释放后用双相系统直接回收细胞内生物分子。在这项研究中，通过使用酒精/盐 ABS 的提取破坏从重组马克斯克鲁维酵母细胞中回收虾青素。重组K. marxianus酵母经过精心设计，可产生高浓度的游离虾青素。最高分配系数 ( K  = 90.02 ± 2.25) 和产量 ( Y = 96.80% ± 0.05) 的虾青素是用 ABS 获得的，ABS 由 20% (w/w) 1-丙醇和 20% (w/w) 柠檬酸钠组成，pH 5、0.5% (w/w) 酵母细胞负载和额外的1% (w/w) 1-丁基-3-甲基咪唑鎓四氟硼酸盐 (Bmim)BF ₄以改善虾青素向富含酒精的上相的迁移。在双相系统中加入 2.5 小时的超声波处理进一步提高了 ABS 中虾青素的回收率。超声波辅助酒精/盐 ABS 将虾青素的提取和浓缩集成在一个单步操作中，证明了从重组马克斯 K. marxianus细胞中直接回收虾青素。