Rhizosphere microbial communities are dynamic and play a crucial role in diverse biochemical processes and nutrient cycling. Soil type and cultivar modulate the composition of rhizosphere microbial communities. Changes in the community composition significantly alter microbial function and ecological process. We examined the influence of soil type on eubacterial and diazotrophic community abundance and microbial metabolic potential in chickpea (cv. BG 372 and cv. BG 256) rhizosphere. The total eubacterial and diazotrophic community as estimated through 16 S rDNA and nifH gene copy numbers using qPCR showed the soil type influence with clear rhizosphere effect on gene abundance. PLFA study has shown the variation in microbial community structure with different soil types. Differential influence of soil types and cultivar on the ratio of Gram positive to Gram negative bacteria was observed with most rhizosphere soils corresponding to higher ratios than bulk soil. The rhizosphere microbial activities (urease, dehydrogenase, alkaline phosphatase and beta-glucosidase) were also assessed as an indicator of microbial metabolic diversity. Principal component analysis and K-means non-hierarchical cluster mapping grouped soils into three categories, each having different soil enzyme activity or edaphic drivers. Soil type and cultivar influence on average substrate utilization pattern analyzed through community level physiological profiling (CLPP) was higher for rhizosphere soils than bulk soils. The soil nutrient studies revealed that both soil type and cultivar influenced the available N, P, K and organic carbon content of rhizosphere soil. Our study signifies that soil type and cultivar jointly influenced soil microbial community abundance and their metabolic potential in chickpea rhizosphere.

根际微生物群落是动态的，在不同的生化过程和养分循环中起着至关重要的作用。土壤类型和栽培品种调节根际微生物群落的组成。群落组成的变化显着改变了微生物功能和生态过程。我们研究了土壤类型对鹰嘴豆（cv. BG 372 和 cv. BG 256）根际中真细菌和固氮群落丰度和微生物代谢潜力的影响。通过 16 S rDNA 和nifH估计的总真细菌和固氮菌群落使用 qPCR 的基因拷贝数显示土壤类型对基因丰度的影响具有明显的根际效应。PLFA 研究表明，不同土壤类型的微生物群落结构存在差异。观察到土壤类型和品种对革兰氏阳性菌与革兰氏阴性菌比率的不同影响，大多数根际土壤对应于比散装土壤更高的比率。根际微生物活性（脲酶、脱氢酶、碱性磷酸酶和β-葡萄糖苷酶）也被评估为微生物代谢多样性的指标。主成分分析和 K 均值非层次聚类映射将土壤分为三类，每类具有不同的土壤酶活性或土壤驱动因素。通过群落水平生理分析 (CLPP) 分析的土壤类型和栽培品种对根际土壤的平均基质利用模式的影响高于散装土壤。土壤养分研究表明，土壤类型和栽培品种都会影响根际土壤的有效氮、磷、钾和有机碳含量。我们的研究表明土壤类型和品种共同影响了鹰嘴豆根际土壤微生物群落丰度及其代谢潜力。