Circ-E2F3 promotes cervical cancer progression by inhibiting microRNA-296-5p and increasing STAT3 nuclear translocation,Annals of the New York Academy of Sciences

当前位置： X-MOL 学术 › Ann. N. Y. Acad. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Circ-E2F3 promotes cervical cancer progression by inhibiting microRNA-296-5p and increasing STAT3 nuclear translocation
Annals of the New York Academy of Sciences ( IF 4.1 ) Pub Date : 2021-09-01 , DOI: 10.1111/nyas.14653
Xiangke Cao ₁ , Qinghua Ma ₂ , Bin Wang ₃ , Qingqiang Qian ₄ , Yinan Xi ₅

Affiliation

Circular RNA E2F transcription factor 3 (circ-E2F3) has been demonstrated to be differentially expressed in some diseases and cancers. However, the role of circ-E2F3 in cervical cancer (CC) progression remains unclear. Therefore, we aimed to elucidate the mechanism of circ-E2F3 regulation of CC progression. Circ-E2F3 expression was determined in CC samples, and its correlation with the clinicopathological characteristics of CC patients and cell biological processes was examined. The interaction among circ-E2F3, microRNA-296-5p (miR-296-5p), and signal transducer and activator of transcription 3 (STAT3) was analyzed by dual luciferase reporter gene and fluorescence in situ hybridization assays. Circ-E2F3–depleted CaSki cells were implanted into nude mice to verify the function of circ-E2F3 in vivo. Circ-E2F3 was upregulated in both CC tissues and cell lines, and this correlated with the clinicopathological features and poor prognosis of CC patients. Moreover, circ-E2F3 promoted the proliferation, invasion, and migration of CC cells and tumor growth in vivo. It was also observed that circ-E2F3 promoted the nuclear translocation of STAT3 through inhibition of miR-296-5p, thus affecting the expression of cyclin D1. Taken together, the key findings of our study demonstrate that circ-E2F3 induces inhibition of miR-296-5p, which triggers activation and nuclear translocation of STAT3 that then upregulates cyclin D1 expression.

中文翻译：

Circ-E2F3 通过抑制 microRNA-296-5p 和增加 STAT3 核转位促进宫颈癌进展

环状 RNA E2F 转录因子 3 (circ-E2F3) 已被证明在某些疾病和癌症中差异表达。然而，circ-E2F3 在宫颈癌 (CC) 进展中的作用仍不清楚。因此，我们旨在阐明 circ-E2F3 调控 CC 进展的机制。测定 CC 样本中 Circ-E2F3 的表达，并检查其与 CC 患者的临床病理特征和细胞生物学过程的相关性。通过双荧光素酶报告基因和荧光原位杂交试验分析了 circ-E2F3、microRNA-296-5p (miR-296-5p) 和信号转导和转录激活因子 3 (STAT3) 之间的相互作用。将去除 Circ-E2F3 的 CaSki 细胞植入裸鼠体内以验证 circ-E2F3在体内的功能. Circ-E2F3 在 CC 组织和细胞系中均上调，这与 CC 患者的临床病理特征和不良预后相关。此外，circ-E2F3促进了CC细胞的增殖、侵袭和迁移以及体内肿瘤的生长。还观察到circ-E2F3通过抑制miR-296-5p促进STAT3的核转位，从而影响cyclin D1的表达。总之，我们研究的主要发现表明 circ-E2F3 诱导 miR-296-5p 的抑制，从而触发 STAT3 的激活和核转位，然后上调 cyclin D1 的表达。

更新日期：2021-09-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11