To improve understanding of the role of Ralstonia in cystic fibrosis (CF), whole genomes of 18 strains from clinical samples were sequenced using Illumina technology. Sequences were analysed by core genome Multi-Locus Sequence Typing, Average Nucleotide Identity based on BLAST (ANIb), RAST annotation, and by ResFinder. Phylogenetic analysis was performed for the 16S rRNA gene, and the OXA-22 and OXA-60 ß-lactamase families. The minimal inhibitory concentrations (MICs) were determined using broth microdilution. ANIb data for the 18 isolates and 54 strains from GenBank, supported by phylogenetic analysis, showed that 8 groups of clusters (A-H), as well as subgroups that should be considered as species or subspecies. Groups A-C contain strains previously identified as Ralstonia solanacearum and Ralstonia pseudosolanacearum. We propose that group A is a novel species. Group B and C are Ralstonia syzygii, Ralstonia solanacearum, respectively. Group D is composed of Ralstonia mannitolilytica and Group E of Ralstonia pickettii. Group F and G should be considered novel species. Group H strains belong to R. insidiosa. OXA-22 and OXA-60 family ß-lactamases were encoded by all strains. Co-trimoxazole generally showed high activity with low MICs (≤1 mg/l) as did ciprofloxacin (≤0.12 mg/l). MICs against the other antibiotics were more variable, but generally high. RAST annotation revealed limited differences between the strains, and virulence factors were not identified. The taxonomy of the genus Ralstonia is in need of revision, but sequencing additional isolates is needed. Antibiotic resistance levels are high. Annotation did not identify potential virulence factors.

为了更好地了解Ralstonia在囊性纤维化 (CF) 中的作用，使用 Illumina 技术对来自临床样本的 18 个菌株的全基因组进行了测序。通过核心基因组多位点序列分型、基于 BLAST (ANIb) 的平均核苷酸同一性、RAST 注释和 ResFinder 分析序列。对 16S rRNA 基因以及 OXA-22 和 OXA-60 β-内酰胺酶家族进行了系统发育分析。使用肉汤微量稀释法测定最小抑制浓度 (MIC)。在系统发育分析的支持下，来自 GenBank 的 18 个分离物和 54 个菌株的 ANIb 数据显示，8 组簇 (AH) 以及应被视为种或亚种的亚组。AC组包含先前鉴定为Ralstonia solanacearum的菌株和Ralstonia pseudosolanacearum。我们认为A组是一个新物种。B组和C组分别是Ralstonia syzygii、Ralstonia solanacearum。D组由Ralstonia mannitolilytica和Ralstonia pickettii E组组成。F组和G组应被视为新物种。H组菌株属于R. insidiosa. OXA-22 和 OXA-60 家族 ß-内酰胺酶由所有菌株编码。复方新诺明通常表现出高活性和低 MIC (≤1 mg/l)，环丙沙星 (≤0.12 mg/l) 也是如此。针对其他抗生素的 MIC 变化较大，但普遍较高。RAST 注释显示菌株之间的差异有限，并且未鉴定毒力因子。Ralstonia属的分类学需要修订，但需要对其他分离株进行测序。抗生素耐药性水平很高。注释没有确定潜在的毒力因素。