Inhibition of HIV-1 protease (PR) activity is realized by exposure to ⁶⁰Co γ-radiation. The radiation effects on enzyme kinetics of HIV-1 PR are subsequently monitored using nanopore sensor. Substantial loss of proteolytic efficiency towards GagPol polypeptide is observed due to the radiation treatment. Results shows ∼50% of GagPol polypeptide was not involved in HIV-1 PR proteolysis by exposure to ultra-low intensity of γ-radiation (0.1K Gy), and the values reach to over 90% with high γ-ray treatment. Besides, the inactivation effect is also verified in blood samples which contain the virus protease. Our finding provides the potential benefits of γ-radiation to inactivate viral proteinic function, and might be a complementary to the designation of HIV-1 PR inhibitors.

HIV-1 蛋白酶 (PR) 活性的抑制是通过暴露于⁶⁰ Co γ-辐射来实现的。随后使用纳米孔传感器监测辐射对 HIV-1 PR 酶动力学的影响。由于辐射处理，观察到对 GagPol 多肽的蛋白水解效率的显着损失。结果表明，在超低强度γ射线（0.1K Gy）照射下，约50%的GagPol多肽不参与HIV-1 PR蛋白水解，在高γ射线照射下，该值达到90%以上。此外，在含有病毒蛋白酶的血液样本中也验证了灭活效果。我们的发现提供了 γ 辐射灭活病毒蛋白质功能的潜在好处，并且可能是对 HIV-1 PR 抑制剂名称的补充。