Dual Role of Phenyl Amides from Hempseed on BACE 1, PPARγ, and PGC-1α in N2a-APP Cells,Journal of Natural Products

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Dual Role of Phenyl Amides from Hempseed on BACE 1, PPARγ, and PGC-1α in N2a-APP Cells
Journal of Natural Products ( IF 3.3 ) Pub Date : 2021-08-30 , DOI: 10.1021/acs.jnatprod.1c00435
Julio Rea Martinez ₁ , Gordana Šelo ₂ , María Ángeles Fernández-Arche ₁ , Beatriz Bermudez ₃ , María Dolores García-Giménez ₁

Affiliation

In Alzheimer’s disease (AD) the accumulation of amyloid β (Aβ) plaques in the brain leads to neuroinflammation, neuronal cell dysfunction, and progressive memory loss. Therefore, blocking the formation of Aβ plaques has emerged as one of the most promising strategies to develop AD treatments. Hempseed is widely used as a food, and recently its compounds have shown beneficial effects on neuroinflammation. The objective of this study was to investigate whether a fraction rich in phenyl amide compounds, N-trans-caffeoyltyramine (CAFT) and N-trans-coumaroyltyramine (CUMT), can affect gene expression: β-site amyloid-precursor-protein-cleaving enzyme 1 (BACE 1), peroxisome proliferator-activated receptor gamma (PPAR γ), and PPARγ-coactivator-1α (PGC-1α) in N2a-APP cells. The mRNA levels were measured using RT-qPCR. The ethyl acetate fraction and CAFT were found to reduce BACE1 gene expression and are promissory PPARγ and PGC-1α natural agonists. The results show that hempseed compounds can inhibit the expression of BACE 1, which is involved in the accumulation of Aβ plaques and positively affect transcription factors involved in complex and diverse biological functions.

中文翻译：

大麻籽中的苯酰胺对 N2a-APP 细胞中 BACE 1、PPARγ 和 PGC-1α 的双重作用

在阿尔茨海默病 (AD) 中，大脑中β淀粉样蛋白 (Aβ) 斑块的积累会导致神经炎症、神经元细胞功能障碍和进行性记忆丧失。因此，阻断 Aβ 斑块的形成已成为开发 AD 治疗最有前途的策略之一。大麻籽被广泛用作食品，最近其化合物显示出对神经炎症的有益作用。本研究的目的是研究富含苯酰胺化合物N -反式- 咖啡酰胺 (CAFT) 和N -反式- 香豆酰胺 (CUMT) 的级分是否可以影响基因表达：β 位点淀粉样蛋白前体蛋白裂解N2a-APP 细胞中的酶 1 (BACE 1)、过氧化物酶体增殖物激活受体 γ (PPAR γ) 和 PPARγ-辅激活剂-1α (PGC-1α)。使用 RT-qPCR 测量 mRNA 水平。乙酸乙酯组分和 CAFT 被发现可降低 BACE1 基因表达，并且是 PPARγ 和 PGC-1α 天然激动剂。结果表明，大麻籽化合物可以抑制 BACE 1 的表达，BACE 1 参与 Aβ 斑块的积累，并对参与复杂多样的生物功能的转录因子产生积极影响。

更新日期：2021-09-24

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