Abstract

Curcumin and Zinc oxide nanoparticle (ZnO-NP) are known as organic and mineral wound healing agents, respectively. In this study, we aimed to green-synthesize the curcumin-mediated ZnO-NP (CmZnO-NP) and evaluate biological properties. In this study, CmZnO-NP were synthesized and after characterizing (DLS, XRD, FT IR, FESEM) its antioxidant effects (ABTS, DPP H), toxicity (MTT) and wound healing (H&E staining and gene expression) were investigated. The 30.77-nm CmZnO-NP significantly scavenged both free radicals. Also, its wound healing activity was observed by detecting the meaningful reduction in the length and depth of the rat’s skin wound. The collagen and VEGF up-regulation of the rat skin tissue following the increasing CmZnO-NP treatment doses confirmed its wound healing potential. Due to antioxidant and improved wound healing activities of the CmZnO-NP, it has the potential to be studied as an efficient wound healing compound.

摘要

姜黄素和氧化锌纳米颗粒 (ZnO-NP) 分别被称为有机和矿物伤口愈合剂。在这项研究中，我们旨在绿色合成姜黄素介导的 ZnO-NP (CmZnO-NP) 并评估生物学特性。在本研究中，合成了 CmZnO-NP，并在表征（DLS、XRD、FT IR、FESEM）后研究了其抗氧化作用（ABTS、DPP H）、毒性（MTT）和伤口愈合（H&E 染色和基因表达）。30.77 nm CmZnO-NP 显着清除了两种自由基。此外，通过检测大鼠皮肤伤口的长度和深度的有意义的减少来观察其伤口愈合活性。在增加 CmZnO-NP 治疗剂量后，大鼠皮肤组织的胶原蛋白和 VEGF 上调证实了其伤口愈合潜力。