Abstract

Nigella sativa oil [NSO] was enriched with biosynthesized silver nanoparticles [NP] by simple, cost-effective, and less time-consuming “Green Chemistry” protocol. This combination [NSO/NP], on blending with PVA, produced transparent and foldable hydrogel film. The structure, morphology, hydrophilicity and thermal stability of hydrogel film confirmed the incorporation of inorganic NP in organic PVA matrix. FTIR spectrum of nanocomposite film revealed good interactions between PVA matrix, NP and NSO. SEM of PVA and NSO combination film exhibited lipidic NSO cloud-like phase (light grey) dispersed in PVA matrix (dark contrast), while in nanocomposite hydrogel film these lipidic clouds disappeared completely, and PVA matrix appeared donning a grey sheath. The nanocomposite film showed good folding endurance (> 300 folds) and antimicrobial behavior against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATTC 29212), Escherichia coli (ATCC 26922), Pseudomonas aeruginosa (ATCC 27853), Proteus mirabilis (ATCC 29906), Klebsiella pneumonia (MTCC109), Acinetobacter baumannii (NCTC 12156), and Candida albicans (ATCC90028) with prospective applications in antimicrobial packaging and wound dressings.

摘要

Nigella sativa oil [NSO] 通过简单、经济且耗时的“绿色化学”方案富含生物合成的银纳米颗粒 [NP]。这种组合 [NSO/NP] 在与 PVA 混合时，产生透明且可折叠的水凝胶薄膜。水凝胶膜的结构、形貌、亲水性和热稳定性证实了无机NP在有机PVA基质中的掺入。纳米复合薄膜的 FTIR 光谱显示 PVA 基质、NP 和 NSO 之间具有良好的相互作用。PVA和NSO组合膜的SEM显示脂质NSO云状相（浅灰色）分散在PVA基质中（暗对比），而在纳米复合水凝胶膜中这些脂质云完全消失，PVA基质出现灰色鞘。纳米复合薄膜表现出良好的耐折性（>金黄色葡萄球菌(ATCC 25923)、粪肠球菌(ATTC 29212)、大肠杆菌(ATCC 26922)、铜绿假单胞菌(ATCC 27853)、奇异变形杆菌(ATCC 29906)、肺炎克雷伯菌( MTCC109)、鲍曼不动杆菌(NCTC 12156)白色念珠菌（ATCC90028）在抗菌包装和伤口敷料中具有前瞻性应用。