As biochemical oxygen demand (BOD) properly illustrates transformation of toxic organic compounds to harmless and biodegradable substances, it is often used to demonstrate the efficiency of wastewater treatment technologies. Nevertheless, H₂O₂ can form when solutions are subjected to advanced oxidation processes (AOP). H₂O₂ production is typical in high energy irradiation treatments. Toxic effect of H₂O₂ on microbes is a well known fact in the field of biology, however, the impact of H₂O₂ is usually not taken into account when evaluating effectiveness of AOP. To quantify the impact of the forming H₂O₂ on the outcome of BOD tests, the BOD of glucose-glutamic acid reference solution was determined in the presence of hydrogen peroxide in the 0.1 and 75 mg dm⁻³ concentration range. Results showed that H₂O₂ distorts the BOD measurement in two ways. Prolonged lag period takes place that ensures adaptation of microbes to toxic environment and promotes production of catalase enzyme that decomposes hydrogen peroxide. On the other hand, appearance of catalase producing bacterial groups leads to reduced BOD values because the oxygen released from the H₂O₂ results in a negative error as it acts against oxygen depletion. The correlation between H₂O₂ concentration and the shift in lag period was described, as well as the relationship between the initial H₂O₂ concentration and the final BOD results. To validate the effects studied, BOD was measured in 0.1 mmol dm⁻³ oxacillin solutions irradiated with gamma rays. It was confirmed that the presence of H₂O₂ may lead to underestimation of the actual BOD as increase in BOD was observed when hydrogen peroxide was eliminated from the solution before starting the measurement.

由于生化需氧量 (BOD) 恰当地说明了有毒有机化合物向无害且可生物降解的物质的转化，因此它通常用于证明废水处理技术的效率。然而，当溶液经受高级氧化过程 (AOP) 时，会形成H ₂ O ₂。H ₂ O _{2 的}产生在高能辐射处理中是典型的。H ₂ O ₂对微生物的毒性作用在生物学领域是众所周知的事实，但是在评估AOP的有效性时通常不考虑H ₂ O ₂的影响。量化形成 H ₂ O 的影响₂关于 BOD 测试的结果，葡萄糖 - 谷氨酸参考溶液的 BOD 在 0.1 和 75 mg dm ^-3浓度范围内的过氧化氢存在下测定。结果表明，H ₂ O ₂以两种方式扭曲了BOD 测量值。发生延长的滞后期，以确保微生物适应有毒环境并促进分解过氧化氢的过氧化氢酶的产生。另一方面，产生过氧化氢酶的细菌群的出现会导致 BOD 值降低，因为从 H ₂ O ₂释放的氧气会导致负误差，因为它会对抗氧气消耗。H ₂ O ₂之间的相关性描述了浓度和滞后期的变化，以及初始 H ₂ O ₂浓度和最终 BOD 结果之间的关系。为了验证所研究的效果，在用伽马射线照射的0.1 mmol dm ^-3苯唑西林溶液中测量 BOD 。已经证实，H ₂ O ₂的存在可能导致实际BOD 的低估，因为在开始测量之前从溶液中去除过氧化氢时观察到BOD 增加。