Abstract

Water-soluble isonicotinamide-stabilized fluorescent gold nanoclusters (AuNCs) were synthesized in one step and used as fluorescent probes for the sensitive and selective determination of 2,4,6-trinitrophenol (TNP). The AuNCs were characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and fluorescence spectroscopy. The fluorescence of the isonicotinamide-stabilized AuNCs was quenched by TNP that follows a linear relationship with the TNP concentration from 0.2 to 20 µM. The sensor provides a low detection limit of 0.06 µM. By using absorption spectroscopy, fluorescence spectroscopy and fluorescence lifetime, the fluorescence quenching mechanism was demonstrated to be due to the inner filter effect between AuNCs and TNP. The sensor was successfully employed for TNP determination in water samples.

摘要

一步合成水溶性异烟酰胺稳定的荧光金纳米簇 (AuNCs)，并将其用作荧光探针，用于灵敏和选择性测定 2,4,6-三硝基苯酚 (TNP)。通过透射电子显微镜、X射线光电子能谱、傅里叶变换红外光谱和荧光光谱对AuNCs进行了表征。异烟酰胺稳定的 AuNC 的荧光被 TNP 淬灭，TNP 与 0.2 至 20 μM 的 TNP 浓度呈线性关系。该传感器提供 0.06 µM 的低检测限。通过使用吸收光谱、荧光光谱和荧光寿命，证明了荧光猝灭机制是由于AuNCs和TNP之间的内滤效应。