Background: Enhancer RNA (eRNA) is a non-coding RNA transcribed by enhancers and has a crucial role in controlling gene transcription. However, the potential functions of eRNA in prostate cancer (PCa) are still not fully understood.

Methods: Clinical information and expression data of eRNAs and their target genes for PCa were downloaded from TCGA. Each eRNA in PCa samples was divided into high- and low-expression groups. The Kaplan–Meier method was used to identify the eRNAs significantly associated with the overall survival of PCa. Their correlations with age, prostate-specific antigen (PSA), and tumor stage were analyzed using Spearman coefficients. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEEG) analyzes were used to predict the possible biological functions and pathways associated with eRNAs.

Results: We identified 12 eRNAs associated with PCa survival, of which PARGP1 was the most clinically significant. It was associated with biochemical recurrence in PCa patients and was also a valuable complement to distinguish between PSA 0–4 ng/ml and 4–10 ng/ml. GO, and KEEG analysis showed that herpes simplex virus-1 infection was the most biologically significant enriched process.

Conclusions: PARGP1 is associated with biochemical recurrence in prostate cancer and contributes to assessing the outcome.

背景：增强子 RNA (eRNA) 是一种由增强子转录的非编码 RNA，在控制基因转录中起着至关重要的作用。然而，eRNA 在前列腺癌 (PCa) 中的潜在功能仍未完全了解。

方法：从TCGA下载eRNAs及其PCa靶基因的临床信息和表达数据。PCa 样本中的每个 eRNA 被分为高表达组和低表达组。Kaplan-Meier 方法用于鉴定与 PCa 总生存显着相关的 eRNA。使用 Spearman 系数分析它们与年龄、前列腺特异性抗原 (PSA) 和肿瘤分期的相关性。最后，使用基因本体论 (GO) 和京都基因和基因组百科全书 (KEEG) 分析来预测与 eRNA 相关的可能的生物学功能和途径。

结果：我们确定了 12 种与 PCa 存活相关的 eRNA，其中 PARGP1 具有最显着的临床意义。它与 PCa 患者的生化复发有关，也是区分 PSA 0-4 ng/ml 和 4-10 ng/ml 的有价值的补充。GO 和 KEEG 分析表明，单纯疱疹病毒 1 感染是最具生物学意义的富集过程。

结论： PARGP1 与前列腺癌的生化复发相关，有助于评估结果。