ABSTRACT

Objective

To investigate the histopathological effects of a peripheral benzodiazepine receptor agonist (Ro5-4864) on epidural fibrosis (EF) in an experimental study model (post-laminectomy) in rats.

Methods

A total of 32 albino Wistar rats were randomly divided into four equal groups (n = 8). In Group 1, no treatment was applied after laminectomy (control group). In Group 2, hemostasis was achieved after Laminectomy, and the surgical procedure was terminated by placing a 2-mm absorbable gelatin sponge dipped in saline into the epidural space. In Group 3, low-dose (4 mg/kg) Ro5-4864 was administered 30 minutes before the surgery. In Group 4, high-dose (8 mg/kg) Ro5-4864 was administered 30 minutes before the surgery. A histopathological examination was performed to evaluate arachnoidal invasion and EF.

Results

Our data revealed the EF was significantly reduced in rats treated with high-dose Ro5-4864 (Group 4) compared to the control and saline-soaked Spongostan groups (p = 0.000 and p = 0.006, respectively). There was no significant difference between the groups treated with high- and low-dose Ro5-4864. Arachnoidal invasion was not seen in any of the rats in the high-dose R05-4864 group. However, the arachnoidal invasion results did not significantly differ between the study groups (p = 0.052 = 0.05).

Conclusions

Our study showed that Ro5-4864 could be effective in reducing EF in rats after.

中文翻译：

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Ro5-4864（外周苯二氮卓受体激动剂）对大鼠椎板切除术后脊髓硬膜外纤维化的预防作用

摘要

客观的

在大鼠实验研究模型（椎板切除术后）中研究外周苯二氮卓受体激动剂 (Ro5-4864) 对硬膜外纤维化 (EF) 的组织病理学影响。

方法

总共 32 只白化 Wistar 大鼠被随机分为四个相等的组 (n = 8)。第一组椎板切除术后不进行任何治疗（对照组）。在第 2 组中，椎板切除术后止血，通过将浸有盐水的 2 mm 可吸收明胶海绵放入硬膜外腔终止手术过程。在第 3 组中，在手术前 30 分钟给予低剂量 (4 mg/kg) Ro5-4864。第 4 组在手术前 30 分钟给予高剂量 (8 mg/kg) Ro5-4864。进行组织病理学检查以评估蛛网膜浸润和 EF。

结果

我们的数据显示，与对照组和盐水浸泡的 Spongostan 组相比，用高剂量 Ro5-4864（第 4 组）治疗的大鼠的 EF 显着降低（分别为 p = 0.000 和 p = 0.006）。用高剂量和低剂量 Ro5-4864 治疗的组之间没有显着差异。高剂量R05-4864组大鼠均未见蛛网膜浸润。然而，研究组之间的蛛网膜侵入结果没有显着差异（p = 0.052 = 0.05）。

发现

我们的研究表明，Ro5-4864 可有效降低大鼠术后 EF。