Defect Induced Charge Redistribution and Enhanced Adsorption of Lysozyme on Hydroxyapatite for Efficient Antibacterial Activity,Langmuir

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Defect Induced Charge Redistribution and Enhanced Adsorption of Lysozyme on Hydroxyapatite for Efficient Antibacterial Activity
Langmuir ( IF 3.7 ) Pub Date : 2021-08-31 , DOI: 10.1021/acs.langmuir.1c01666
Ming Wang ₁ , Yingchun Zhu _{1,

2}

Affiliation

Defects in hydroxyapatite (HA) have attracted increasing research interest due to their significant functions to increase the bioactivity and antibacterial ability of hard-tissue implants. However, little is known about the natural property and functional mechanism of the defects in HA. Herein, we reported on the defect property concerned with the coordination state and charge distribution in Al doped HA, as well as the consequent interface and protein capture ability for improved antibacterial activity. Systemic investigations suggested that Al replacing Ca in HA induced coordination defect with decreased coordination number and bond distance, caused charge transfer and redistribution of surrounding O atom and resulted in an increase in negative charge of coordinated O atoms. These O atoms coordinated with Al further served as docking sites for lysozyme molecules via electrostatic and H-bonding interaction. The capacity of lysozyme adsorption for Al-HA increased approximately 10-fold more than that of HA, which significantly increased the antibacterial activity through lysozyme-catalyzed splitting of cell wall of bacteria. Moreover, in vitro studies indicated that Al-HA materials showed good cytocompatibility. These findings not only provided new insights into the important effect of defects on the performances of HA biomaterials by modulation of the coordination state, charge distribution, and chemical activity, but also proposed a promising method for efficient antibacterial activity of HA biomaterials.

中文翻译：

缺陷诱导电荷重新分布和溶菌酶在羟基磷灰石上的增强吸附以实现高效抗菌活性

羟基磷灰石 (HA) 中的缺陷因其在增加硬组织植入物的生物活性和抗菌能力方面的重要作用而引起了越来越多的研究兴趣。然而，人们对HA缺陷的自然特性和功能机制知之甚少。在此，我们报告了与 Al 掺杂的 HA 中的配位状态和电荷分布有关的缺陷特性，以及随之而来的界面和蛋白质捕获能力，以提高抗菌活性。系统研究表明，铝取代 HA 中的 Ca 引起配位缺陷，配位数和键距降低，引起周围 O 原子的电荷转移和重新分布，导致配位 O 原子的负电荷增加。这些与铝配位的 O 原子通过静电和 H 键相互作用进一步充当溶菌酶分子的对接位点。溶菌酶对Al-HA的吸附能力比HA增加约10倍，通过溶菌酶催化的细菌细胞壁分裂显着提高了抗菌活性。此外，体外研究表明，Al-HA 材料显示出良好的细胞相容性。这些发现不仅为缺陷通过调节配位态、电荷分布和化学活性对 HA 生物材料性能的重要影响提供了新的见解，而且为 HA 生物材料的有效抗菌活性提供了一种有前途的方法。溶菌酶对Al-HA的吸附能力比HA增加约10倍，通过溶菌酶催化的细菌细胞壁分裂显着提高了抗菌活性。此外，体外研究表明，Al-HA 材料显示出良好的细胞相容性。这些发现不仅为缺陷通过调节配位态、电荷分布和化学活性对 HA 生物材料性能的重要影响提供了新的见解，而且为 HA 生物材料的有效抗菌活性提供了一种有前途的方法。溶菌酶对Al-HA的吸附能力比HA增加约10倍，通过溶菌酶催化的细菌细胞壁分裂显着提高了抗菌活性。此外，体外研究表明，Al-HA 材料显示出良好的细胞相容性。这些发现不仅为缺陷通过调节配位态、电荷分布和化学活性对 HA 生物材料性能的重要影响提供了新的见解，而且为 HA 生物材料的有效抗菌活性提供了一种有前途的方法。体外研究表明，Al-HA 材料显示出良好的细胞相容性。这些发现不仅为缺陷通过调节配位态、电荷分布和化学活性对 HA 生物材料性能的重要影响提供了新的见解，而且为 HA 生物材料的有效抗菌活性提供了一种有前途的方法。体外研究表明，Al-HA 材料显示出良好的细胞相容性。这些发现不仅为缺陷通过调节配位态、电荷分布和化学活性对 HA 生物材料性能的重要影响提供了新的见解，而且为 HA 生物材料的有效抗菌活性提供了一种有前途的方法。

更新日期：2021-09-14

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