Vindoline is an important alkaloid produced in Catharanthus roseus leaves. It is the more important monomer of the scarce and costly anticancer bisindole alkaloids, vincristine, and vinblastine, as unlike catharanthine (the other monomer), its biosynthesis is restricted to the leaves. Here, biotic (bacterial endophyte, phytoplasma, virus) and abiotic (temperature, salinity, SA, MeJa) factors were studied for their effect on vindoline accumulation in C. roseus. Variations in vindoline pathway-related gene expression were reflected in changes in vindoline content. Since allene oxide cyclase (CrAOC) is involved in jasmonate biosynthesis and MeJa modulates many vindoline pathway genes, the correlation between CrAOC expression and vindoline content was studied. It was taken up for full-length cloning, tissue-specific expression profiling, in silico analyses, and upstream genomic region analysis for cis-regulatory elements. Co-expression analysis of CrAOC with vindoline metabolism-related genes under the influence of aforementioned abiotic/biotic factors indicated its stronger direct correlation with the tabersonine-to-vindoline genes (t16h, omt, t3o, t3r, nmt, d4h, dat) as compared to the pre-tabersonine genes (tdc, str, sgd). Its expression was inversely related to that of downstream-acting peroxidase (prx) (except under temperature stress). Direct/positive relationship of CrAOC expression with vindoline content established it as a key gene modulating vindoline accumulation in C. roseus.

Vindoline是长春花叶子中产生的一种重要的生物碱。它是稀缺且昂贵的抗癌双吲哚生物碱、长春新碱和长春碱中更重要的单体，与长春花碱（另一种单体）不同，它的生物合成仅限于叶子。在这里，研究了生物（细菌内生菌、植原体、病毒）和非生物（温度、盐度、SA、MeJa）因子对C. roseus中长春多林积累的影响。vindoline 通路相关基因表达的变化反映在vindoline 含量的变化中。由于丙二烯氧化物环化酶 ( CrAOC ) 参与茉莉酸的生物合成，而 MeJa 调节许多文多林途径基因，CrAOC之间的相关性表达和vindoline含量进行了研究。它被用于全长克隆、组织特异性表达谱分析、计算机分析和顺式调节元件的上游基因组区域分析。在上述非生物/生物因素的影响下， CrAOC与文多林代谢相关基因的共表达分析表明，它与丁香碱到文多林的基因（ t16h、omt、t3o、t3r、nmt、d4h、dat）的直接相关性更强与 pre-tabersonine 基因 ( tdc , str , sgd ) 相比）。它的表达与下游作用过氧化物酶（ prx ）的表达呈负相关（温度胁迫下除外）。CrAOC表达与vindoline含量的直接/正相关关系确定它是调节C.roseus中vindoline积累的关键基因。