Background Adhatoda vasica L. is a medicinal plant, known as Malabar nut in English, belonging to the family Acanthaceae. It has been used traditionally to treat respiratory disorders like severe coughs, colds, chronic bronchitis, asthma, tuberculosis, and other illnesses. The multifunctional range of bioactives found in it has piqued the interest of pharmaceutical companies, who are looking for more evidence-based ways to develop new formulations. Objective To analyse the A. vasica leaves by GC-MS technique and evaluation of its antioxidant activity. Method Methanolic extract of A. vasica L. (MEAV) leaves was analyzed by GC-MS for identification and characterization of its bioactives and traditional therapeutic claims. A widely anticipated 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used to determine the antioxidant activity of MEAV. Results The major compounds revealed in MEAV leaves are: 1,3,5-triazine-2,4,6-triamine (3.06%); 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (5.35%); 5-hydroxymethylfurfural (16.82%); 2-butylphenol (6.85%); 3,4-dihydroxy-5-methyl-dihydro-furan-2-on (2.5%); 2(OR 3)–(1,1-dimethylethyl)-4-methoxyphenol (3.52%); megastigmatrienone 3 (1.02%); tetradecanoic acid (1.52%); vomifoliol (0.58%); oxalic acid, cyclobutylhexyl ester (6.03%); hexadecanoic acid (6.06%); 4-ethyl-2-oxo-2,5,6,7-tetrahydro-1H-cyclopenta[B]pyridine-3-carbonitrile (10.08%); phytol (2.01%); and vitamin E (3.18%). A significant reduction in free radicals against DPPH was observed, which revealed the antioxidant potential of MEAV. Conclusion MEAV consists of both polar and nonpolar components. GC-MS analysis was used to identify these compounds. The current work validates that the antioxidant activity of MEAV is attributed to the presence of compounds such as vitamin E and terpenes. Highlights This work validates the antioxidant activity of methanolic extract of A. vasica attributed to the presence of compounds like vitamin E and terpenes.

中文翻译：

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气相色谱-质谱法鉴定紫花叶甲醇提取物中的植物成分及其抗氧化活性

背景 Adhatoda vasica L.是一种药用植物，英文称为Malabar nut，属于刺槐科。它传统上用于治疗呼吸系统疾病，如严重咳嗽、感冒、慢性支气管炎、哮喘、肺结核和其他疾病。其中发现的多功能生物活性剂引起了制药公司的兴趣，他们正在寻找更多基于证据的方法来开发新的配方。目的采用GC-MS技术分析白花蛇舌草叶片并评价其抗氧化活性。方法 通过 GC-MS 分析 A. vasica L. (MEAV) 叶子的甲醇提取物，以鉴定和表征其生物活性和传统治疗声称。广泛预期的 2,2-diphenyl-1-picrylhydrazyl (DPPH) 方法用于确定 MEAV 的抗氧化活性。结果 MEAV叶片中主要成分为：1,3,5-三嗪-2,4,6-三胺（3.06%）；2,3-二氢-3,5-二羟基-6-甲基-4H-吡喃-4-酮（5.35%）；5-羟甲基糠醛（16.82%）；2-丁基苯酚（6.85%）；3,4-二羟基-5-甲基-二氢呋喃-2-on (2.5%)；2(OR 3)-(1,1-二甲基乙基)-4-甲氧基苯酚(3.52%)；megastigmatrienone 3 (1.02%); 十四烷酸（1.52%）；伏米叶醇（0.58%）；草酸，环丁基己酯（6.03%）；十六烷酸（6.06%）；4-乙基-2-氧代-2,5,6,7-四氢-1H-环五[B]吡啶-3-甲腈（10.08%）；植物醇（2.01%）；和维生素 E (3.18%)。观察到针对 DPPH 的自由基显着减少，这揭示了 MEAV 的抗氧化潜力。结论 MEAV 由极性和非极性成分组成。GC-MS 分析用于鉴定这些化合物。目前的工作证实 MEAV 的抗氧化活性归因于维生素 E 和萜烯等化合物的存在。亮点 这项工作验证了由于维生素 E 和萜烯等化合物的存在而产生的 A. vasica 甲醇提取物的抗氧化活性。