The spatial organization of the nucleus is a key determinant in all genome activities. However, the accurate measurement of the nuclear organization is still technically challenging. Here, the technology NucQuant we created previously was utilized to detect the variation of the nuclear organization, including the heterogeneity of the nuclear geometry, the change of the NPC distribution along different cell cycle stages during interphase, and the organization of the nucleolus. The results confirmed that not only the growth rate and the NPC distribution are influenced by the carbon source; the nuclear shape is also impacted by the carbon source. The nuclei lost their spherical geometry gradually when the cell was cultured from the most to a less favorable carbon source. We also discovered that the nucleolus prefers to locate at the nuclear periphery, which was called the “genes poor region,” especially when the cells entered quiescence. Furthermore, the distribution of the NPC along the different stages during the interphase was analyzed. We proposed that with the growth of the cell, the nucleus would grow from the surface of the NE flanking the nucleolus firstly.

中文翻译：

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高分辨率显微镜了解活酵母细胞的核组织


细胞核的空间组织是所有基因组活动的关键决定因素。然而，核组织的精确测量在技术上仍然具有挑战性。在这里，我们利用之前创建的技术 NucQuant 来检测核组织的变化，包括核几何形状的异质性、间期不同细胞周期阶段的 NPC 分布变化以及核仁的组织。结果证实，碳源不仅影响生长速度和NPC分布，而且影响生长速度。核形状也受到碳源的影响。当细胞从最有利的碳源培养到最不利的碳源时，细胞核逐渐失去其球形几何形状。我们还发现，核仁更喜欢位于核外围，这被称为“基因贫乏区”，尤其是当细胞进入静止状态时。此外，分析了间期期间NPC沿不同阶段的分布。我们提出，随着细胞的生长，细胞核将首先从核仁侧翼的NE表面生长。