Currently, in vitro cancer cell culture technology is very important for cancer research. Existing studies have shown that two-dimensional (2D) cell environment may cause significant differences from tumor cells in vivo, resulting in high failure rates when the therapies developed in 2D tumor models translated to the clinic. And compared with 2D culture, the isotropic three-dimensional (3D) culture can construct an environment more similar to that in the body. Here, we utilized 3D printed porous microgel to culture for lung cancer cell and also explored possible mechanism of how 3D culture environment regulates actin cytoskeleton. Compared with 2D cultured lung cancer cells, cells in porous microgel are more similar to those extracted from the existing gold standard: mouse transplanted tumors, in terms of the ROCK-actin pathway. In addition, this study also revealed that ROCK pathway altered by environment played an important role in regulating the drug sensitivity of lung cancer cells. Hence, this 3D printed porous microgel is a promising lung cancer cell culture method, which shows potentional application in future cancer research and drug development.

目前，体外癌细胞培养技术对癌症研究非常重要。现有研究表明，二维（2D）细胞环境可能与体内肿瘤细胞产生显着差异，导致在 2D 肿瘤模型中开发的疗法转化为临床时的高失败率。并且与2D文化相比，各向同性的3D（3D）文化可以构建更接近于身体的环境。在这里，我们利用 3D 打印的多孔微凝胶培养肺癌细胞，并探索了 3D 培养环境如何调节肌动蛋白细胞骨架的可能机制。与 2D 培养的肺癌细胞相比，多孔微凝胶中的细胞更类似于从现有的金标准中提取的细胞：小鼠移植的肿瘤，就 ROCK-肌动蛋白通路而言。此外，本研究还揭示了环境改变的ROCK通路在调节肺癌细胞的药物敏感性中发挥了重要作用。因此，