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Enzymatic activity and physico-chemical changes of terminal bud in rain-fed fig (Ficus carica L. ‘Sabz’) during dormant season
Horticultural Plant Journal ( IF 5.7 ) Pub Date : 2021-08-30 , DOI: 10.1016/j.hpj.2021.03.010
Sahar Sedaghat 1 , Badii Gaaliche 2 , Majid Rahemi 1 , Hamid Zare 3 , Moslem Jafari 3
Affiliation  

Rain-fed fig culture is known to provide a good quality of dried figs, while almost no research has been reported so far on the enzyme activity comparisons of seasonal variations among terminal buds in this plant. Toward this goal, we evaluated the physiochemical changes of terminal buds of fig 'Sabz' and their micromorphology during the dormant season (November to April) using Scanning Electron Microscope (SEM) analysis during two consecutive years (2016 and 2017). Results indicated that the highest contents of total sugars in terminal fig buds were registered in January and the lowest values were noticed in April. SEM analysis showed the presence of vegetative terminal fig buds during the dormant season and after bud-break in April. An increasing trend was observed for the protein content, length and diameter of terminal buds at the beginning of bud-break. In dormant buds, ascorbate peroxidase, peroxidase and catalase enzymes exhibited initially an ascending pattern, while it decreased from December. A second increase for these enzymes occurred during the dormant season until bud-break. Superoxide dismutase activity increased in buds during the dormancy season until bud-break. Proline content remained stable in buds from November to February, while it decreased rapidly until bud-break. Furthermore, a downward trend of the total phenol content in buds was observed between the onset and end of dormant period. In general, the dormant buds in ʻSabzʼ fig cultivar were metabolically active with a short dormant period under the rain-fed conditions.



中文翻译:

雨养无花果(Ficus carica L. 'Sabz')休眠期顶芽酶活性及理化变化

众所周知,雨养无花果栽培可提供优质的无花果干,而迄今为止几乎没有关于这种植物末端芽之间的酶活性比较季节性变化的研究报道。为实现这一目标,我们连续两年(2016 年和 2017 年)使用扫描电子显微镜 (SEM) 分析评估了无花果‘Sabz’ 顶芽在休眠季节(11 月至 4 月)的理化变化及其微形态。结果表明,无花果末梢总糖含量在1月份最高,4月份最低。扫描电镜分析显示无花果在休眠期和 4 月萌芽后存在无花果的营养终端芽。蛋白质含量呈上升趋势,萌芽开始时顶芽的长度和直径。在休眠芽中,抗坏血酸过氧化物酶、过氧化物酶和过氧化氢酶最初呈现上升模式,而从12月开始下降。这些酶的第二次增加发生在休眠季节直到萌芽。在休眠季节直到萌芽期间,芽中的超氧化物歧化酶活性增加。11月至2月芽中脯氨酸含量保持稳定,但在萌芽前迅速下降。此外,在休眠期开始和结束之间观察到芽中总酚含量呈下降趋势。一般来说,‘Sabz’无花果品种的休眠芽在雨养条件下代谢活跃,休眠期短。过氧化物酶和过氧化氢酶最初呈现上升模式,而从 12 月开始下降。这些酶的第二次增加发生在休眠季节直到萌芽。在休眠季节直到萌芽期间,芽中的超氧化物歧化酶活性增加。11月至2月芽中脯氨酸含量保持稳定,但在萌芽前迅速下降。此外,在休眠期开始和结束之间观察到芽中总酚含量呈下降趋势。一般来说,‘Sabz’无花果品种的休眠芽在雨养条件下代谢活跃,休眠期短。过氧化物酶和过氧化氢酶最初呈现上升模式,而从 12 月开始下降。这些酶的第二次增加发生在休眠季节直到萌芽。在休眠季节直到萌芽期间,芽中的超氧化物歧化酶活性增加。11月至2月芽中脯氨酸含量保持稳定,但在萌芽前迅速下降。此外,在休眠期开始和结束之间观察到芽中总酚含量呈下降趋势。一般来说,‘Sabz’无花果品种的休眠芽在雨养条件下代谢活跃,休眠期短。在休眠季节直到萌芽期间,芽中的超氧化物歧化酶活性增加。11月至2月芽中脯氨酸含量保持稳定,但在萌芽前迅速下降。此外,在休眠期开始和结束之间观察到芽中总酚含量呈下降趋势。一般来说,‘Sabz’无花果品种的休眠芽在雨养条件下代谢活跃,休眠期短。在休眠季节直到萌芽期间,芽中的超氧化物歧化酶活性增加。11月至2月芽中脯氨酸含量保持稳定,但在萌芽前迅速下降。此外,在休眠期开始和结束之间观察到芽中总酚含量呈下降趋势。一般来说,‘Sabz’无花果品种的休眠芽在雨养条件下代谢活跃,休眠期短。

更新日期:2021-08-30
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