Plant pathogens resistant to the commercially available fungicides and bactericides even at higher concentrations are the biggest challenge for the farmers to control the losses due to plant diseases. The antibacterial and antifungal potential of nanomaterials makes them a suitable candidate for the control of plant diseases. Thus, the present study reports the phytofabricated zinc oxide nanoparticles (ZnO Np’s) using aqueous plant leaf extract of Terminalia bellerica (Baheda). Characterization of ZnO nanoparticles was done by ultraviolet–visible (UV–Vis) studies, X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infra-red (FT–IR) analysis, and transmission electron microscopy (TEM). The presence of pure hexagonal wurtzite crystalline structure of ZnO nanoparticles was confirmed by XRD analysis. The TEM images revealed the spherical to hexagonal shaped ZnO nanoparticles with sizes ranging from 20 to 30 nm. The stabilization of synthesized ZnO nanoparticles through the interactions of terpenoids, steroids, phenylpropanoids, flavonoids, phenolic acids, and enzymes present in the leaf extract was suggested by FTIR analysis. The mechanism of the formation of ZnO nanoparticles using Terminalia bellerica (Baheda) (Tb-ZnO Np’s) as a bioactive compound is proposed. These phytofabricated ZnO nanoparticles (Tb-ZnO Np’s) have shown significant antifungal potential against Alternaria brassicae the causal agent of Alternaria blight disease/leaf spot disease in Brassica species. The microscopic results confirm the changes in mycelium morphology and reduction in the number of spore germination at 0.2 mg/mL concentration Tb-ZnO Np’s.

即使在较高浓度下，对市售杀菌剂和杀菌剂也具有抗性的植物病原体是农民控制因植物病害造成的损失的最大挑战。纳米材料的抗菌和抗真菌潜力使其成为控制植物病害的合适候选者。因此，本研究报告了使用榄仁树的水性植物叶提取物的植物制造的氧化锌纳米颗粒 (ZnO Np's)（巴赫达）。通过紫外-可见 (UV-Vis) 研究、X 射线衍射 (XRD)、扫描电子显微镜 (SEM)、傅里叶变换红外 (FT-IR) 分析和透射电子显微镜 (TEM) 对 ZnO 纳米粒子进行表征）。XRD分析证实了ZnO纳米颗粒的纯六方纤锌矿晶体结构的存在。TEM 图像显示球形至六角形 ZnO 纳米颗粒，尺寸范围为 20 至 30 nm。FTIR 分析表明，通过叶提取物中存在的萜类、类固醇、苯丙烷、类黄酮、酚酸和酶的相互作用，合成的 ZnO 纳米颗粒具有稳定性。利用榄仁形成 ZnO 纳米颗粒的机理(Baheda) (Tb-ZnO Np's) 作为一种生物活性化合物被提出。这些植物制造的 ZnO 纳米颗粒 (Tb-ZnO Np's) 已显示出显着的抗真菌潜力，可对抗链格孢菌，它是芸苔属物种中链格孢疫病/叶斑病的病原体。显微结果证实了在 0.2 mg/mL 浓度 Tb-ZnO Np 时菌丝体形态的变化和孢子萌发数量的减少。