Abstract

The use of Raman spectroscopy combined with saliva is an exciting emerging spectroscopy-biofluid combination. In this review, we summarize current methods employed in such studies, in particular the collection, pretreatment, and storage of saliva, as well as measurement procedures and Raman parameters used. Given the need for sensitive detection, surface-enhanced Raman methods are also surveyed, alongside chemometric techniques. A meta-analysis of variables is compiled. We observe a wide range of approaches and conclude that standardization of methods and progress to more extensive validation Raman-saliva studies is necessary. Nevertheless, the studies show tremendous promise toward the improvement of speed, diagnostic accuracy, and portable device possibilities in applications such as healthcare, law enforcement, and forensics.

摘要

将拉曼光谱与唾液结合使用是一种令人兴奋的新兴光谱-生物流体组合。在这篇综述中，我们总结了目前此类研究中采用的方法，特别是唾液的收集、预处理和储存，以及使用的测量程序和拉曼参数。鉴于对灵敏检测的需求，还研究了表面增强拉曼方法以及化学计量技术。编制了变量的荟萃分析。我们观察了广泛的方法并得出结论，方法的标准化和更广泛的验证拉曼唾液研究的进展是必要的。尽管如此，这些研究表明，在提高速度、诊断准确性和便携式设备在医疗保健、执法和取证等应用中的可能性方面具有巨大的前景。