Mechanobiological conceptual framework for assessing stem cell bioprocess effectiveness,Biotechnology and Bioengineering

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Mechanobiological conceptual framework for assessing stem cell bioprocess effectiveness
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2021-08-30 , DOI: 10.1002/bit.27929
Mee-Hae Kim ₁ , Masahiro Kino-Oka ₁

Affiliation

Fully realizing the enormous potential of stem cells requires developing efficient bioprocesses and optimizations founded in mechanobiological considerations. Here, we emphasize the importance of mechanotransduction as one of the governing principles of stem cell bioprocesses, underscoring the need to further explore the behavioral mechanisms involved in sensing mechanical cues and coordinating transcriptional responses. We identify the sources of intrinsic, extrinsic, and external noise in bioprocesses requiring further study, and discuss the criteria and indicators that may be used to assess and predict cell-to-cell variability resulting from environmental fluctuations. Specifically, we propose a conceptual framework to explain the impact of mechanical forces within the cellular environment, identify key cell state determinants in bioprocesses, and discuss downstream implementation challenges.

中文翻译：

评估干细胞生物过程有效性的机械生物学概念框架

充分实现干细胞的巨大潜力需要开发基于机械生物学考虑的高效生物过程和优化。在这里，我们强调机械转导作为干细胞生物过程的控制原则之一的重要性，强调需要进一步探索涉及感知机械线索和协调转录反应的行为机制。我们确定了生物过程中需要进一步研究的内在、外在和外部噪声的来源，并讨论了可用于评估和预测由环境波动引起的细胞间变异性的标准和指标。具体来说，我们提出了一个概念框架来解释机械力在细胞环境中的影响，识别生物过程中的关键细胞状态决定因素，

更新日期：2021-08-30

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