Nan'ao Golden Scallop, Chlamys nobilis, is an important scallop species in aquaculture and is rich in lipids and carotenoid. Previous studies have shown that microalgae diet has a significant effect on the accumulation of total lipid content (TLC) and total carotenoid content (TCC) in bivalves. However, the effects of single-species microalgae diet on accumulation of TLC and TCC at molecular level are unclear. In this study, we used C. nobilis as a bivalve animal model to assess the effects of single-species microalgae diet (Tetraselmis subcordiformis, Dicrateria zhanjiangensis and Chaetoceros mulleri) on the accumulation of lipids and carotenoids, and the expression of stearoyl-CoA desaturase (SCD) and scavenger receptor class B-like-3 (SRB-like-3) genes in C. nobilis. The results of this study indicated that high lipid microalgae diet promoted the accumulation of TCC in C. nobilis tissues. The expression of SCD and SRB-like-3 genes was highly affected by the lipid composition of diet, in which high expression of SCD and SRB-like-3 genes was correlated with low C16:1n-9 and/or C18:1n-9t and/or C16:1/C16:0 and/or C18:1/C18:0. In intestine and adductor muscle, the expression of SCD gene was positively correlated with accumulation of TCC, TLC and fatty acid content, suggesting that SCD gene may indirectly promote the accumulation of TCC (lipid soluble) by promoting lipid accumulation. For SRB-like-3 gene, the high expression of SRB-like-3 was only correlated to the high TCC in intestine, suggesting that SRB-like-3 may be a candidate gene for carotenoid absorption in C. nobilis and has nothing to do with the transport or accumulation of carotenoid. The findings of this study are of great significance for further understanding how microalgae diet affects the accumulation of TCC and TLC, and the expression of lipid-related genes in bivalves.

中文翻译：

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单种微藻饲料对南澳金扇贝脂质和类胡萝卜素积累及脂质相关基因表达的影响

南澳金扇贝（Chlamys nobilis）是水产养殖中重要的扇贝品种，富含脂质和类胡萝卜素。以往的研究表明，微藻饮食对双壳类动物总脂质含量（TLC）和总类胡萝卜素含量（TCC）的积累有显着影响。然而，单种微藻饮食对分子水平上 TLC 和 TCC 积累的影响尚不清楚。在这项研究中，我们使用C. nobilis作为双壳类动物模型来评估单种微藻饮食（Tetraselmis subcordiformis、Dicrateria zhanjiangensis和Chaetoceros mulleri）对脂质和类胡萝卜素积累以及硬脂酰辅酶A去饱和酶表达的影响(SCD ) 和清道夫受体 B-like-3 ( SRB - like - 3 ) 基因在C. nobilis中。本研究结果表明，高脂微藻饮食促进了 TCC 在C. nobilis组织中的积累。SCD和SRB - like - 3基因的表达 受日粮脂质成分的影响很大，其中SCD和SRB - like - 3 基因的高表达与低C16:1n-9和/或C18:1n-相关9t 和/或 C16:1/C16:0 和/或 C18:1/C18:0。在肠和内收肌中，表达SCD基因与TCC、TLC和脂肪酸含量的积累呈正相关，提示SCD基因可能通过促进脂质积累间接促进TCC（脂溶性）的积累。对于SRB -like - 3基因 ，SRB -like - 3 的高表达仅与肠道TCC高相关，提示SRB -like - 3可能 是C. nobilis 类胡萝卜素吸收的候选基因与类胡萝卜素的运输或积累无关。本研究结果对于进一步了解微藻饮食如何影响TCC和TLC的积累以及双壳类脂质相关基因的表达具有重要意义。