Current Eye Research ( IF 1.7 ) Pub Date : 2021-08-29 , DOI: 10.1080/02713683.2021.1963785 Maryam Ghaffari Laleh 1 , Mortaza Bonyadi 1 , Elham Shahriyari 1 , Mohammad Hossein Jabbarpoor Bonyadi 2 , Masoud Soheilian 2 , Mehdi Yaseri 3
ABSTRACT
Purpose
This case-control study aimed to evaluate the possible association of MCP-1 − 2518A/G genetic polymorphism with Behcet’s disease (BD) in the Iranian patients.
Materials and methods
This study was performed in 135 Behcet’s patients (51 ocular and 84 non-ocular) and 79 healthy individuals. Peripheral blood samples were genotyped for MCP-1 − 2518A/G using the PCR-RFLP technique.
Results
The statistical analysis of MCP-1 − 2518A/G showed no significant differences in genotype/allele frequencies between Behcet’s patients and controls. There was no significant association in genotype/allele frequencies between either ocular or non-ocular BD patients and controls. Also, different genotype/allele frequencies between ocular and non-ocular BD were not statistically significant.
Conclusions
In this study, with a threshold P-value of 0.05 and an estimated power of 0.81 to detect a significant association (odds ratio ≥1.2), we did not observe any association of this variant with Behcet's disease.
中文翻译:
单核细胞趋化蛋白 1 (MCP-1) 基因启动子多态性与伊朗人群有和没有眼部受累的白塞病之间缺乏关联:病例对照研究
摘要
目的
本病例对照研究旨在评估伊朗患者中 MCP-1 - 2518A/G 基因多态性与白塞病 (BD) 的可能关联。
材料和方法
这项研究是在 135 名 Behcet 病患者(51 名眼病患者和 84 名非眼病患者)和 79 名健康个体中进行的。使用 PCR-RFLP 技术对外周血样本进行 MCP-1 - 2518A/G 基因分型。
结果
MCP-1 - 2518A/G 的统计分析显示 Behcet 患者和对照组之间的基因型/等位基因频率没有显着差异。眼部或非眼部BD患者与对照之间的基因型/等位基因频率没有显着关联。此外,眼部和非眼部BD之间的不同基因型/等位基因频率没有统计学意义。
结论
在这项研究中,使用 0.05 的阈值P值和 0.81 的估计功效来检测显着关联(优势比 ≥1.2),我们没有观察到这种变体与白塞病的任何关联。