Microbial rhodopsins have advanced optogenetics since the discovery of channelrhodopsins almost two decades ago. During this time an abundance of microbial rhodopsins has been discovered, engineered, and improved for studies in neuroscience and other animal research fields. Optogenetic applications in plant research, however, lagged largely behind. Starting with light-regulated gene expression, optogenetics has slowly expanded into plant research. The recently established all-trans retinal production in plants now enables the use of many microbial opsins, bringing extra opportunities to plant research. In this review, we summarize the recent advances of rhodopsin-based plant optogenetics and provide a perspective for future use, combined with fluorescent sensors to monitor physiological parameters.

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视紫红质光遗传学在植物研究中的进展与展望


自从近二十年前发现通道视紫红质以来，微生物视紫红质已经推动了光遗传学的发展。在此期间，大量的微生物视紫红质被发现、改造和改进，用于神经科学和其他动物研究领域的研究。然而，光遗传学在植物研究中的应用却大大落后。从光调控基因表达开始，光遗传学逐渐扩展到植物研究领域。最近在植物中建立的全反式视网膜生产现在可以使用许多微生物视蛋白，为植物研究带来额外的机会。在这篇综述中，我们总结了基于视紫红质的植物光遗传学的最新进展，并为未来结合荧光传感器监测生理参数提供了前景。