Abstract

Biotechnological methods are an essential component of plant genetic resources investigation that allow both to preserve a rare natural plant and useful selection genotypes, especially essential oil bearing plants used in medicine, perfume, food, etc. In the clonal micropropagation in vitro, the key moment is to retain genetic stability of in vitro material. It is considered that plant regeneration in vitro from meristem or vegetative buds gives identical clones, but the effect of the medium growth regulators on the genetic stability of in vitro plant material is discussed. Therefore, the objective of our work was to evaluate the genetic similarity of ex situ and in vitro plants. Investigation was performed on Hyssopus officinalis L. cv. Nikitskiy Beliy (NBG selection). Regeneration from the shoot single-node segments on the modified Murashige and Skoog culture medium (MS) supplemented with 6-benzylaminopurine (BAP) in concentration 0.3–0.9 mg/L and 0.1 mg/L Indole-3-butyric acid (IBA) was carried out. It was established, that application of 0.5 mg/L BAP was sufficient for the organ formation and development without morphological deviations. Genetic analysis based on RAPD and ISSR-PCR had shown full genetic similarity of the investigated ex situ and in vitro plants.

中文翻译：

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Hyssopus officinalis L.的体外再生与植物遗传相似性

摘要

生物技术方法是植物遗传资源调查的重要组成部分，既可以保存稀有的天然植物，也可以选择有用的基因型，特别是用于医药、香水、食品等的含精油植物。在体外克隆微繁中，关键时刻是保持体外材料的遗传稳定性。认为从分生组织或营养芽体外再生植物得到相同的克隆，但讨论了培养基生长调节剂对离体植物材料遗传稳定性的影响。因此，我们工作的目的是评估异地植物和离体植物的遗传相似性。对Hyssopus officinalis进行了调查L.简历。Nikitskiy Beliy（NBG 选择）。在补充有浓度为 0.3-0.9 mg/L 和 0.1 mg/L Indole-3-butyric acid (IBA) 的 6-苄氨基嘌呤 (BAP) 的改良 Murashige 和 Skoog 培养基 (MS) 上从枝条单节段再生是执行。已经确定，应用 0.5 mg/L BAP 足以使器官形成和发育而没有形态学偏差。基于 RAPD 和 ISSR-PCR 的遗传分析显示了所研究的异地植物和体外植物的完全遗传相似性。