The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has infected millions of people worldwide. SARS-CoV-2 belongs to the Betacoronavirus genus, containing the mouse hepatitis virus (MHV), an extensively studied animal coronavirus. Since MHV and SARS-CoV-2 share the same genus, MHV could offer insights relative to SARS-CoV-2 studies. MHV-3 strain causes hepatitis and cellular injury, making MHV-3 infection one of the best models for this debilitating disease. Surrogate coronaviruses have been used for virus resistance and inactivation studies, and although real‐life conditions using SARS-CoV-2 should be encouraged, their use needs to be balanced with safety and costs. MHV can be manipulated under BSL2 laboratory conditions, unlike SARS-CoV-2, making it a model for studying the virucidal effects on coronaviruses. In this study, we used the betacoronavirus MHV-3 as a model to investigate the virucidal activity of an air disinfection equipment named STR Solution®, an air sterilizer with patented technology. MHV-3 was dried on different surfaces and exposed at varying distances from the STR Solution® equipment and at different exposure times. The residual infectivity was evaluated using the endpoint method. There was not a significant reduction (mean p-value = 0.4) of the viral titer under STR Solution® exposition. STR Solution® caused a slight decrease of the infectious particles’ titer (> 1 log₁₀) only under the following conditions: polypropylene at 3 m, for 1 and 3 h (1.2 log₁₀ reduction TCID₅₀) and Sus domesticus skin at 0.05 m, for 1 h (1.3 log₁₀ reduction TCID50), and at 3 m for 1 h (1.2 log₁₀ reduction TCID₅₀). These and other studies confirm the usefulness of this model to evaluate virucidal activity.

严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 已感染全球数百万人。SARS-CoV-2 属于Beta冠状病毒属，包含小鼠肝炎病毒 (MHV)，这是一种广泛研究的动物冠状病毒。由于 MHV 和 SARS-CoV-2 属于同一属，因此 MHV 可以提供与 SARS-CoV-2 研究相关的见解。MHV-3 毒株会引起肝炎和细胞损伤，使 MHV-3 感染成为治疗这种衰弱性疾病的最佳模型之一。替代冠状病毒已用于病毒抗性和灭活研究，尽管应鼓励在现实生活条件下使用 SARS-CoV-2，但它们的使用需要与安全性和成本相平衡。与 SARS-CoV-2 不同，MHV 可以在 BSL2 实验室条件下进行操作，使其成为研究对冠状病毒杀病毒作用的模型。在这项研究中，我们使用 β 冠状病毒 MHV-3 作为模型来研究名为 STR Solution® 的空气消毒设备的杀病毒活性，具有专利技术的空气消毒器。MHV-3 在不同的表面上干燥，并在与 STR Solution® 设备的不同距离和不同的曝光时间下曝光。使用终点法评估残留感染性。在 STR Solution® 暴露下，病毒滴度没有显着降低（平均 p 值 = 0.4）。STR Solution® 导致传染性颗粒滴度略有下降（> 1 log₁₀ ) 仅在以下条件下：聚丙烯在 3 m，持续 1 和 3 小时（1.2 log ₁₀减少 TCID ₅₀）和Sus domesticus皮肤在 0.05 m，持续 1 小时（1.3 log ₁₀减少 TCID50），在 3 m 1 小时（1.2 log ₁₀减少 TCID ₅₀）。这些和其他研究证实了该模型对评估杀病毒活性的有用性。