Microchimica Acta ( IF 5.3 ) Pub Date : 2021-08-27 , DOI: 10.1007/s00604-021-04965-0 Fan Yang 1, 2 , Linlin Yang 1, 3 , Longchang Xu 1 , Wei Guo 1, 2 , Lei Pan 1, 2 , Chuanglin Zhang 1 , Shihao Xu 1, 4 , Liang Yang 1, 4 , Changlong Jiang 1, 4 , Ningning Zhang 5
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Abstract
A portable smartphone device is reported that uses 3D printing technology for the primary diagnosis of diseases by detecting acetone. The key part of the device consists of red carbon dots (RCDs), which are used as internal standards, and a sensing reagent (3-N,N-(diacethydrazide)-9-ethylcarbazole (2-HCA)) for acetone. With an excitation wavelength of 360 nm, the emission wavelengths of 2-HCA and RCDs are 443 nm and 619 nm, respectively. 2-HCA effectively captures acetone to form a nonfluorescent acylhydrazone via a condensation reaction occurring in aqueous solution, resulting in obvious color changes from blue-violet to dark red. The detection limit for acetone is 2.62 μM (~ 0.24 ppm). This is far lower than the ketone content in normal human blood (≤ 0.50 mM) and the acetone content in human respiratory gas (≤ 1.80 ppm). The device has good recovery rates for acetone detection in blood and exhaled breath, which are 90.56–109.98% (RSD ≤ 5.48) and 92.80–108.00% (RSD ≤ 5.07), respectively. The method designed here provides a reliable way to provide health warnings by visually detecting markers of ketosis/diabetes in blood or exhaled breath.
Graphical abstract
中文翻译：
基于智能手机的 3D 打印设备，通过丙酮响应染料标记和红色发射碳点对酮症进行荧光诊断
摘要
据报道，一种便携式智能手机设备使用 3D 打印技术通过检测丙酮对疾病进行初步诊断。该装置的关键部分由用作内标的红碳点 (RCD) 和传感试剂 (3- N,N-(diacethydrazide)-9-ethylcarbazole (2-HCA)) 为丙酮。激发波长为 360 nm，2-HCA 和 RCD 的发射波长分别为 443 nm 和 619 nm。2-HCA通过在水溶液中发生的缩合反应有效地捕获丙酮形成无荧光的酰腙，导致明显的颜色从蓝紫色变为深红色。丙酮的检测限为 2.62 μM (~ 0.24 ppm)。这远低于正常人体血液中的酮含量（≤ 0.50 mM）和人体呼吸气体中的丙酮含量（≤ 1.80 ppm）。该装置对血液和呼出气中的丙酮检测具有良好的回收率，分别为 90.56-109.98%（RSD ≤ 5.48）和 92.80-108.00%（RSD ≤ 5.07）。
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