Effect of low temperature and low oxygen concentration on biochemical attributes, antioxidant enzyme activities, phenolics profile and expression of phenylpropanoid pathway-related genes was investigated in crabapple (Malus profusion) fruit. Crabapple fruit were kept at room temperature (RT, 20 ± 2 °C and 25 ± 2 °C alternatively at 12 h cycle) or low temperature (LT, 10 ± 2 °C and 25 ± 2 °C alternatively at 12 h cycle) and supplemented with either normal oxygen (21%) or low oxygen levels (5%) for 9 days. Results revealed higher concentrations of soluble solids, titratable acids, and ascorbic acid in fruit stored at LT + 5% O₂. Likewise, total phenolics, peroxidase activity, and 2,2´-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activity were observed to be significantly higher in fruit stored at low temperature (LT + 5% O₂ > LT + 21% O₂), followed by those stored at room temperature (RT + 5% O₂ > RT + 21% O₂). In contrast, H₂O₂ and malondialdehyde contents were significantly enhanced in fruit stored at room temperature (RT + 21% O₂ and RT + 5% O₂), whereas fruit stored at low temperature showed minimum inhibition in superoxide dismutase, catalase and ascorbate peroxidase activities. In addition, low temperature induced biosynthesis of phenolic acids, whereas no considerable changes were observed in flavanols and dihydrochalcones during storage. Low temperature and oxygen conditions (LT + 5% O₂) significantly enhanced the biosynthesis of flavonols (glycosylated quercetin derivatives) and cyanidin 3-galactoside, compared to LT + 21% O₂, RT + 5% O₂ and RT + 21% O₂ conditions. In addition, low temperature also up-regulated the expression of MpFLS and MpUFGT in fruit tissues. Moreover, correlation analysis suggested positive association of antioxidant capacity with the biosynthesis of flavonols and anthocyanins in fruit stored at LT + 5% O₂.

低温和生物化学属性低的氧浓度，抗氧化酶活性，酚类谱和苯丙素途径相关的基因表达的影响在海棠（研究了苹果丛生）的水果。将海棠果实保存在室温（RT，20±2°C 和 25±2°C 交替，12 小时循环）或低温（LT，10±2°C 和 25±2°C 交替，12 小时循环）并补充正常氧气 (21%) 或低氧气水平 (5%) 9 天。结果表明，在 LT + 5% O _{2 条件下}储存的水果中可溶性固体、可滴定酸和抗坏血酸的浓度更高. 同样，在低温（LT + 5% O ₂  > LT + 21% O ₂ )，然后是在室温下储存的那些 (RT + 5% O ₂  > RT + 21% O ₂ )。与此相反，H ₂ ö ₂和丙二醛含量在果实中显著增强在室温下储存（RT + 21％氧气₂和RT + 5％氧气₂)，而低温储存的水果对超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶活性的抑制最小。此外，低温诱导酚酸的生物合成，而在储存期间黄烷醇和二氢查耳酮没有观察到显着变化。与 LT + 21% O ₂、RT + 5% O ₂和 RT + 21%相比，低温和氧气条件（LT + 5% O ₂）显着增强了黄酮醇（糖基化槲皮素衍生物）和花青素 3-半乳糖苷的生物合成O ₂条件。此外，低温还上调了MpFLS和MpUFGT的表达在果实组织中。此外，相关性分析表明，在 LT + 5% O _{2 条件下}储存的水果中，抗氧化能力与黄酮醇和花青素的生物合成呈正相关。