Analysis of type I osteoporosis animal models using synchrotron radiation,Microscopy Research and Technique

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Analysis of type I osteoporosis animal models using synchrotron radiation
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-08-28 , DOI: 10.1002/jemt.23911
Subok Kim ₁ , Sanghun Jang ₂ , Jihyeon Ahn ₃ , Sukjun Lee ₄ , Onseok Lee _{1,

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Affiliation

Preclinical experiments to analyze the trabecular space of spongy bones using small animals are required for the evaluation and treatment of patients with osteoporosis (OP). We performed ovariectomy to create OP models. A total of four mice were used. Ovariectomized group (OVX, n = 2) in which both ovaries were resected at random, and the sham operated group (SHAM, n = 2) performed surgery without resecting the ovaries. We propose a study that enables OP analysis by analyzing tibia microstructures of OVX and SHAM using synchrotron radiation (SR). SR imaging is a technology capable of irradiating an extremely small object in the order of several tens of nanometers using a nondestructive method at the microscopic level. Unlike previous imaging diagnoses (staining, micro-CT [Computed Tomography]) it was possible to preserve the real shape and analyze bone microstructures in real-time and analyze and evaluate spongy bones to secure data and increase the reliability of OP analysis. We were able to confirm the possibility of OP diagnosis through experimental animals for spongy bone damage related to bone mineral density. Therefore, we aimed to provide a rehabilitation and medicine therapy intervention method through basic research on the evaluation of OP diagnosis through human-based segmentation of challenging spongy bones while supplementing the limitations of existing imaging methods.

中文翻译：

使用同步加速器辐射分析 I 型骨质疏松症动物模型

评估和治疗骨质疏松症 (OP) 患者需要使用小动物分析海绵骨小梁间隙的临床前实验。我们进行了卵巢切除术以创建 OP 模型。总共使用了四只小鼠。随机切除双侧卵巢的去卵巢组 (OVX, n = 2) 和假手术组 (SHAM, n = 2) 在不切除卵巢的情况下进行了手术。我们提出了一项研究，通过使用同步辐射 (SR) 分析 OVX 和 SHAM 的胫骨微结构来进行 OP 分析。SR 成像是一种能够使用微观级别的非破坏性方法照射几十纳米数量级的极小物体的技术。与以前的影像诊断（染色、显微 CT [计算机断层扫描]）不同，它可以保留真实形状并实时分析骨骼微结构，并分析和评估海绵状骨骼以确保数据并提高 OP 分析的可靠性。我们能够通过与骨矿物质密度相关的海绵状骨损伤的实验动物确认 OP 诊断的可能性。所以，

更新日期：2021-08-28

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