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Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography
Bioactive Materials ( IF 18.0 ) Pub Date : 2021-08-28 , DOI: 10.1016/j.bioactmat.2021.08.028
Huaiyu Shi 1, 2 , Xiangjun Wu 1, 2 , Shiyang Sun 1, 2 , Chenyan Wang 1, 2 , Zacharias Vangelatos 3 , Ariel Ash-Shakoor 1 , Costas P Grigoropoulos 3 , Patrick T Mather 4 , James H Henderson 1, 2 , Zhen Ma 1, 2
Affiliation  

Focal adhesion complexes function as the mediators of cell-extracellular matrix interactions to sense and transmit the extracellular signals. Previous studies have demonstrated that cardiomyocyte focal adhesions can be modulated by surface topographic features. However, the response of focal adhesions to dynamic surface topographic changes remains underexplored. To study this dynamic responsiveness of focal adhesions, we utilized a shape memory polymer-based substrate that can produce a flat-to-wrinkle surface transition triggered by an increase of temperature. Using this dynamic culture system, we analyzed three proteins (paxillin, vinculin and zyxin) from different layers of the focal adhesion complex in response to dynamic extracellular topographic change. Hence, we quantified the dynamic profile of cardiomyocyte focal adhesion in a time-dependent manner, which provides new understanding of dynamic cardiac mechanobiology.



中文翻译:


分析人心肌细胞粘着斑对细胞外动态纳米形貌的反应



粘着斑复合物充当细胞-细胞外基质相互作用的介体,以感知和传递细胞外信号。先前的研究表明,心肌细胞粘着斑可以通过表面形貌特征进行调节。然而,粘着斑对动态表面形貌变化的响应仍有待探索。为了研究粘着斑的动态响应性,我们使用了基于形状记忆聚合物的基材,该基材可以通过温度升高触发产生平坦到皱纹的表面转变。使用这种动态培养系统,我们分析了来自粘着斑复合物不同层的三​​种蛋白质(桩蛋白、纽蛋白和zyxin)对动态细胞外拓扑变化的反应。因此,我们以时间依赖性方式量化了心肌细胞粘着斑的动态特征,这为动态心脏力学生物学提供了新的理解。

更新日期:2021-08-28
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