Functional imaging of intact taste cells in response to various tastant solutions poses a technical challenge since the refractive index of the immersion medium dynamically changes during tastant delivery. Critically, the focal shift introduced by high-index tastant solutions has been the fundamental limit in experimental design. Here we seek to address this issue by introducing an axially elongated Bessel beam in two-photon microscopy. Compared to the conventional Gaussian beam, the Bessel beam provides superior robustness to the index-induced focal shift, allowing us to acquire near artifact-free imaging of taste cells in response to a physiological taste stimulus.

中文翻译：

---

使用贝塞尔光束对味觉进行稳健的功能成像

响应各种促味剂溶液的完整味觉细胞的功能成像提出了技术挑战，因为浸入介质的折射率在促味剂递送过程中会发生动态变化。至关重要的是，高指数味觉解决方案引入的焦点转移一直是实验设计的基本限制。在这里，我们试图通过在双光子显微镜中引入轴向拉长的贝塞尔光束来解决这个问题。与传统的高斯光束相比，贝塞尔光束对指数引起的焦移提供了卓越的鲁棒性，使我们能够响应生理味觉刺激获得几乎无伪影的味觉细胞成像。