The continuous change of the drug landscape in the United States demands adaptation and incorporation of emerging analytical methods that preferably allow onsite screening but are also capable of supporting the analysis of seized drugs received at forensic laboratories across the country. Current methods employing color testing require the interaction of chemical reagents with powdered and liquid materials and can often yield inconclusive results, especially when the material is impure.

Implementation of portable Raman spectroscopy can remove the need for direct interaction with solid and liquid specimens. In this study a portable, 785 nm, Raman spectroscopy system was employed for screening of seized drug samples, including mixtures. First, a panel of analytes comprised of 15 common drugs of abuse, 15 diluents, and 64 different mixtures comprising various ratios of analytes were used to measure bias, precision, and repeatability in accordance with United Nations Office on Drugs and Crime (UNODC) guidelines for handheld Raman field identification devices for seized material. Accuracy and precision through glass packaging was 91% and 90%; and through plastic was 89% and 88%, respectively for the diluents examined. A subset of the pure and mixture samples was then analyzed using direct analysis in real time mass spectrometry (DART-MS). Identification of analytes was performed manually by observing the [M+H]⁺ protonated molecule and conducting a library search of an in-house database. Using DART-MS, the drug analyte present in the sample was correctly identified 92% of the time using the library search feature. The presence of dimers and –OH losses were also observed for many of the analyte drugs of abuse. The combination of portable Raman spectroscopy and DART-MS data resulted in an overall accuracy of 96% for the detection of both drugs and diluents. The combined accuracy when analyzing authentic case samples was 83%, providing a rapid and accurate method for seized drug screening within drug chemistry laboratories.

美国毒品形势的不断变化需要适应和整合新兴的分析方法，这些方法最好允许现场筛查，但也能够支持对全国法医实验室收到的缉获毒品的分析。当前采用颜色测试的方法需要化学试剂与粉末和液体材料的相互作用，并且通常会产生不确定的结果，尤其是当材料不纯时。

便携式拉曼光谱的实施可以消除与固体和液体样品直接相互作用的需要。在这项研究中，使用便携式 785 nm 拉曼光谱系统来筛选缉获的药物样品，包括混合物。首先，根据联合国毒品和犯罪问题办公室 (UNODC) 指南，使用由 15 种常见滥用药物、15 种稀释剂和 64 种不同分析物比例的不同混合物组成的分析物组来测量偏差、精确度和可重复性用于检获材料的手持式拉曼场识别设备。通过玻璃封装的准确度和精密度分别为 91% 和 90%；对于所检测的稀释剂，透塑料率分别为 89% 和 88%。然后使用实时质谱直接分析 (DART-MS) 分析纯样品和混合物样品的子集。通过观察 [M+H] 手动进行分析物的鉴定⁺质子化分子并对内部数据库进行库搜索。使用 DART-MS，使用库搜索功能在 92% 的时间内正确识别样品中存在的药物分析物。对于许多滥用的分析物药物，也观察到二聚体和 -OH 损失的存在。便携式拉曼光谱和 DART-MS 数据的结合使药物和稀释剂检测的总体准确度达到 96%。分析真实病例样本时的综合准确度为 83%，为药物化学实验室内的缉获药物筛查提供了一种快速、准确的方法。