ABSTRACT

Trigonella foenum-graecum L. (fenugreek) leaves are nutritionally rich green leafy vegetables consumed in South-East Asian and Middle-Eastern countries. Scientific data suggests properties including anti-inflammatory, antipyretic, anti-nociception, and hepatoprotective. However, the molecules responsible for these properties are yet to be identified. In the present study, we have isolated and characterized an anti-inflammatory nutraceutical molecule based on bioassay from the fresh leaves of fenugreek. The lipoxygenase inhibition assay revealed 79.9 ± 1.1% and 69.0 ± 2.9% inhibition in ethanolic and aqueous extracts, respectively. The ethanolic extract purified on a semi-prep HPLC column yielded a nutraceutical molecule quercetin glycoside (QG) having potent inhibition of lipoxygenase (75.3 ± 1.6%) at 10 mM and hyaluronidase (67.4 ± 4.0%) at 2 mM, respectively. The QG structure was elucidated by spectroscopic data, including FTIR, UHPLC-MS/MS, and 1D and 2D NMR as Quercetin-3-O-diglucoside-7-O-glucoside. QG also suppressed ROS, NO, and IL-6 production in LPS stimulated RAW264.7 cells. Radical scavenging assays of DPPH and ABTS revealed the potent antioxidant activity of QG with an EC₅₀ of 245.5 and 68.8 μM, respectively. Our results demonstrate that newly characterized quercetin glycoside from fresh leaves of fenugreek possesses potential anti-inflammatory and antioxidant activities.

摘要

芹菜L.（葫芦巴）叶是东南亚和中东国家食用的营养丰富的绿叶蔬菜。科学数据表明其具有抗炎、解热、抗伤害感受和保肝等特性。然而，负责这些特性的分子还有待确定。在本研究中，我们从胡芦巴的新鲜叶子中分离并表征了一种基于生物测定的抗炎营养药物分子。脂肪氧化酶抑制测定显示乙醇和水提取物中分别有 79.9 ± 1.1% 和 69.0 ± 2.9% 的抑制。在半制备型 HPLC 柱上纯化的乙醇提取物产生了一种营养药物分子槲皮素糖苷 (QG)，分别对 10 mM 的脂肪氧化酶 (75.3 ± 1.6%) 和 2 mM 的透明质酸酶 (67.4 ± 4.0%) 具有有效抑制作用。O -diglucoside-7- O -glucoside 。QG 还抑制 LPS 刺激的 RAW264.7 细胞中 ROS、NO 和 IL-6 的产生。DPPH 和 ABTS 的自由基清除试验揭示了 QG 的有效抗氧化活性，EC _{50 分别}为 245.5 和 68.8 μM。我们的结果表明，来自胡芦巴新鲜叶的新特征槲皮素糖苷具有潜在的抗炎和抗氧化活性。