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AAV integration in human hepatocytes
Molecular Therapy ( IF 12.1 ) Pub Date : 2021-08-28 , DOI: 10.1016/j.ymthe.2021.08.031
Dhwanil A Dalwadi 1 , Andrea Calabria 2 , Amita Tiyaboonchai 1 , Jeffrey Posey 1 , Willscott E Naugler 3 , Eugenio Montini 2 , Markus Grompe 1
Affiliation  

Recombinant adeno-associated viral (rAAV) vectors are considered promising tools for gene therapy directed at the liver. Whereas rAAV is thought to be an episomal vector, its single-stranded DNA genome is prone to intra- and inter-molecular recombination leading to rearrangements and integration into the host cell genome. Here, we ascertained the integration frequency of rAAV in human hepatocytes transduced either ex vivo or in vivo and subsequently expanded in a mouse model of xenogeneic liver regeneration. Chromosomal rAAV integration events and vector integrity were determined using the capture-PacBio sequencing approach, a long-read next-generation sequencing method that has not previously been used for this purpose. Chromosomal integrations were found at a surprisingly high frequency of 1%–3% both in vitro and in vivo. Importantly, most of the inserted rAAV sequences were heavily rearranged and were accompanied by deletions of the host genomic sequence at the integration site.



中文翻译:

AAV 在人肝细胞中的整合

重组腺相关病毒 (rAAV) 载体被认为是针对肝脏的基因治疗的有前途的工具。虽然 rAAV 被认为是一种附加型载体,但其单链 DNA 基因组易于发生分子内和分子间重组,导致重排和整合到宿主细胞基因组中。在这里,我们确定了 rAAV 在体外体内转导的人肝细胞中的整合频率随后在异种肝再生的小鼠模型中进行了扩展。使用捕获-PacBio 测序方法确定染色体 rAAV 整合事件和载体完整性,这是一种以前未用于此目的的长读长下一代测序方法。在体外体内均以惊人的 1%–3% 的高频率发现了染色体整合。重要的是,大多数插入的 rAAV 序列被重排,并伴随着整合位点宿主基因组序列的缺失。

更新日期:2021-10-14
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