Biosensors play a central role in moving diagnostics to being on-site or decentralized. Affinity biosensor, an important category of biosensors, has important applications in clinical diagnosis, pharmaceuticals, immunology, and other fields. Affinity biosensors rely on specific binding between target analytes and biological ligands such as antibodies, nucleic acids, or other receptors to generate measurable signals. Oftentimes the target analytes in practical samples are of low abundance in a complex matrix. Traditional affinity biosensors mainly rely on random diffusion of analytes in solution to conjugate with biorecognition elements on the sensor surface of electrodes. The process may take hours or even days, which is not conducive to rapid and sensitive detection of biosensors. Therefore, it is strongly desired to incorporate an enrichment mechanism for target analytes into biosensor-based detection. AC electrokinetic (ACEK) effect can realize rapid enrichment of analytes by application of AC electric fields, which holds great promise for achieving high sensitivity, low detection limit, and rapid turnaround. This article reviews the studies of affinity biosensors integrated with ACEK enrichment in the past decade, and summarizes the latest detection methods, detection devices and applications, hoping to provide some insights and references for researchers in related fields.

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通过集成交流电动富集增强基于亲和力的电分析生物传感器——一个小型回顾

生物传感器在将诊断转移到现场或分散式方面发挥着核心作用。亲和生物传感器是生物传感器的重要类别，在临床诊断、制药、免疫学等领域具有重要应用。亲和生物传感器依赖于目标分析物和生物配体（如抗体、核酸或其他受体）之间的特异性结合来产生可测量的信号。通常实际样品中的目标分析物在复杂基质中的丰度较低。传统的亲和生物传感器主要依靠分析物在溶液中的随机扩散与电极传感器表面的生物识别元件结合。该过程可能需要数小时甚至数天，不利于生物传感器的快速灵敏检测。所以，强烈希望将目标分析物的富集机制结合到基于生物传感器的检测中。交流电动（ACEK）效应可以通过施加交流电场实现分析物的快速富集，这对于实现高灵敏度、低检测限和快速周转具有很大的前景。本文回顾了近十年来与ACEK富集相结合的亲和生物传感器的研究，总结了最新的检测方法、检测装置和应用，希望为相关领域的研究人员提供一些见解和参考。和快速周转。本文回顾了近十年来与ACEK富集相结合的亲和生物传感器的研究，总结了最新的检测方法、检测装置和应用，希望为相关领域的研究人员提供一些见解和参考。和快速周转。本文回顾了近十年来与ACEK富集相结合的亲和生物传感器的研究，总结了最新的检测方法、检测装置和应用，希望为相关领域的研究人员提供一些见解和参考。