This study aimed to explore the effects of recombinant serine protease treatment on the development of post-harvest loquat diseases, fruit quality, and disease resistance enzyme activities. It also sought to analyze differential genes expression using RNA-seq technology. Transcriptomics analysis revealed 708 and 398 differentially expressed genes (DEGs) in loquat fruits treated with serine protease for 24 and 48 h. Furthermore, 2198 DEGs were obtained between 24 and 48 h after treatment. The genes encoding JAZ, MYC2 and ERF in the plant signal transduction pathway were significantly up-regulated. The resistance-related genes, such as PPO, PAL, TLP, WRKY, and transcription factors were also significantly up-regulated. These results indicated that the recombinant serine protease can induce plant signal transduction pathway in loquat fruit. The expression of some resistance-related genes enhanced the disease resistance of loquat fruit and revealed the molecular mechanism of loquat fruit resistance induced by recombinant serine protease.

本研究旨在探讨重组丝氨酸蛋白酶处理对枇杷采后病害发展、果实品质和抗病酶活性的影响。它还试图使用 RNA-seq 技术分析差异基因表达。转录组学分析显示，在用丝氨酸蛋白酶处理 24 和 48 小时的枇杷果实中，有 708 和 398 个差异表达基因 (DEG)。此外，在处理后 24 至 48 小时之间获得了 2198 个 DEG。植物信号转导通路中编码JAZ、MYC2和ERF的基因显着上调。抗性相关基因，如 PPO，PAL、TLP、WRKY 和转录因子也显着上调。这些结果表明重组丝氨酸蛋白酶可以在枇杷果实中诱导植物信号转导途径。部分抗性相关基因的表达增强了枇杷果实的抗病性，揭示了重组丝氨酸蛋白酶诱导枇杷果实抗病性的分子机制。