Background

Cervical-cancer is among the most commonly diagnosed cancers in women, and infection with human papillomavirus (HPV) is associated with an increased risk of cervical cancer and altered serum concentrations of inflammatory cytokines. We have explored the association between a genetic variation in the Interleukin-10 (IL-10) gene (rs1800896) and cervical cancer risk and its relationship with tissue Interferon gamma (IFN-γ), Transforming growth factor beta (TGF-β), Tumor necrosis factor alpha (TNF-α) concentrations in women with cervical cancer.

Methods

A total of 315 women with, or without cervical cancer, were recruited into the study. DNA was extracted from cervical cells, and genotyping was undertaken using Taq-man real-time PCR. The genotype frequency and allele distribution were analyzed together with their association with pathological data. The association of the rs1800896 gene variation with tissue levels of the inflammatory cytokines was also investigated.

Results

Our data showed a significant association between the A allele of the rs1800896 gene variant and the presence of cervical cancer. In particular, patients with AG/AA genotypes had an increased risk of cervical cancer with an odds ratio of 1.929 (95% confidence interval [CI]: 0.879–4.23, P < 0.001) in a recessive model, compared with the GG genotype. Also, the tissue concentrations of IFN-γ, TGF-β, and TNF-α in cervical tissues were significantly higher in women with cervical cancer (P < 0.001) and were associated with the AA genotype.

Conclusion

We have found an association between the polymorphism rs1800896 in the IL-10 gene and an increased risk of cervical cancer as well as a higher level of tissue inflammatory cytokines. Further investigations are necessary on the value of emerging biomarkers for the risk stratification for the management of cervical cancer patients.

中文翻译：

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白细胞介素10基因的遗传变异与宫颈癌相关的风险和炎症增加的关联

背景

宫颈癌是女性最常诊断出的癌症之一，感染人乳头瘤病毒 (HPV) 与宫颈癌风险增加和血清炎症细胞因子浓度改变有关。我们探讨了白细胞介素 10 (IL-10) 基因 (rs1800896) 的遗传变异与宫颈癌风险之间的关联及其与组织干扰素γ (IFN-γ)、转化生长因子β (TGF-β)、宫颈癌女性的肿瘤坏死因子α（TNF-α）浓度。

方法

共有 315 名患有或未患有宫颈癌的女性被招募到该研究中。从宫颈细胞中提取 DNA，并使用 Taq-man 实时 PCR 进行基因分型。分析基因型频率和等位基因分布及其与病理数据的关联。还研究了 rs1800896 基因变异与炎性细胞因子的组织水平的关联。

结果

我们的数据显示 rs1800896 基因变异的 A 等位基因与宫颈癌的存在之间存在显着关联。特别是，与 GG 基因型相比，AG/AA 基因型患者在隐性模型中患宫颈癌的风险增加，优势比为 1.929（95% 置信区间 [CI]：0.879-4.23，P < 0.001）。此外，宫颈癌女性宫颈组织中 IFN-γ、TGF-β 和 TNF-α 的组织浓度显着升高（P < 0.001），并且与 AA 基因型相关。

结论

我们发现 IL-10 基因中的 rs1800896 多态性与宫颈癌风险增加以及组织炎性细胞因子水平升高之间存在关联。需要进一步研究新兴生物标志物对宫颈癌患者管理风险分层的价值。