Bud rot is the most critical disease in Colombian oil palm crops. In addition to implementing current management strategies, it is necessary to search for alternatives to control this disease. This work aimed to assess in vitro the antagonistic activity of 12 isolates of Trichoderma spp. (seven native, three commercial and two donated) against one isolate of Phytophthora palmivora, the causal agent of bud rot. To determine the potential of these isolates in biological control, their competitive abilities, mycoparasitic interactions and the antibiotic properties of their metabolites against P. palmivora in vitro and detached leaf tissue were assessed. The seven native isolates were molecularly identified by partial sequencing of the ITS region and TEF1. As result, in the mycoparasitism tests, coiling, entangling of pathogen hyphae, and colonization of pathogen sporangia were observed. Also, the strain CPTrZC-02 displayed the highest frequency of interactions with pathogen hyphae (55%) and sporangia (63%). For the volatile metabolite activity, inhibition of the diametrical growth of P. palmivora was found, with percentages between 12.8 and 32.2%. For the non-volatile metabolites, the development of P. palmivora was limited, with inhibition percentages between 81 and 98% for isolates CPTrZC-05, CPTrZC-02 and CPTrZC-04. The crude extract from CPTrZC-09 inhibited the development of pathogen lesions at a rate of 100%. The native isolates were identified as Trichoderma reesei (CPTrZC-04), Trichoderma harzianum (CPTrZC-09), Trichoderma asperellum (CPTrZC-05 and CPTrZC-12), and Trichoderma asperelloides (CPTrZC-01, CPTrZC-10, and CPTrZC-11). The isolates T. reesei, T. harzianum, and T. asperelloides, due to their properties, are promising for further field assessments as part of a comprehensive management plan for bud rot in Colombia.

芽腐病是哥伦比亚油棕作物中最严重的病害。除了实施当前的管理策略外，有必要寻找控制这种疾病的替代方法。这项工作旨在评估 12 株木霉属的体外拮抗活性。（七个本地，三个商业和两个捐赠）对抗一种棕榈疫霉分离株，芽腐病的病原体。为了确定这些分离株在生物控制中的潜力，评估了它们的竞争能力、真菌寄生相互作用以及它们的代谢物在体外和分离的叶组织中对抗掌叶枯病菌的抗生素特性。通过对 ITS 区域和 TEF1 进行部分测序，在分子上鉴定了七种天然分离株。结果，在真菌寄生试验中，观察到盘绕、病原体菌丝缠结和病原体孢子囊定植。此外，菌株 CPTrZC-02 与病原体菌丝 (55%) 和孢子囊 (63%) 的相互作用频率最高。对于挥发性代谢物活性，发现对棕榈果蝇的径向生长有抑制作用，百分比在 12.8% 和 32.2% 之间。对于非挥发性代谢物，P. palmivora的开发CPTrZC-05、CPTrZC-02 和 CPTrZC-04 的抑制百分比在 81% 到 98% 之间。CPTrZC-09 的粗提物以 100% 的速率抑制病原体病变的发展。天然分离株被鉴定为里氏木霉(CPTrZC-04)、哈茨木霉(CPTrZC-09)、曲霉木霉(CPTrZC-05 和 CPTrZC-12) 和曲霉木霉(CPTrZC-01、CPTrZC-10、 ）。作为哥伦比亚芽腐病综合管理计划的一部分，分离株里氏木霉、哈茨木霉和黄曲霉，由于它们的特性，有望用于进一步的现场评估。