In the present study, an HPTLC (high-performance thin-layer chromatography) method was developed for the quantitative determination and validation of the curcumin in the methanolic fraction of Curcuma longa L. For achieving good separation of curcumin, the mobile phase of chloroform:methanol (97:3) was used. The densitometric analysis of curcumin was performed at 420 nm in reflection/absorption mode. Linearity of the method was obtained in the range of 100‒600 ng per spot. During analysis, the methanolic fraction of the C. longa showed the presence of a quantifiable amount of curcumin. The content of curcumin was found to be 1.5% (per dry weight). The method is specific, simple, precise, and accurate. The obtained data can have used for the routine analysis of the reported biomarkers in crude drugs and extracts. The quantification and the method validation of curcumin have not yet been reported in C. longa which can be utilized for the proper standardization of the plant.

中文翻译：

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经验证的姜黄甲醇馏分中姜黄素的高效薄层色谱分析

在本研究中，开发了一种 HPTLC（高效薄层色谱）方法，用于定量测定和验证 Curcuma longa L 甲醇馏分中的姜黄素。 为了实现姜黄素的良好分离，氯仿流动相：使用甲醇 (97:3)。姜黄素的光密度分析在 420 nm 处以反射/吸收模式进行。在每个点 100-600 ng 范围内获得了该方法的线性。在分析过程中，C. longa 的甲醇部分显示存在可量化的姜黄素。发现姜黄素的含量为 1.5%（每干重）。该方法专一、简便、精密、准确。所得数据可用于已报道的生药和提取物中生物标志物的常规分析。