Until recently, glycosidases, naturally hydrolyzing carbohydrate-active enzymes, have found few synthetic applications in industry, being primarily used for cleaving unwanted carbohydrates. With the establishment of glycosynthase and transglycosidase technology by genetic engineering, the view of glycosidases as industrial biotechnology tools has started to change. Their easy production, affordability, robustness, and substrate versatility, added to the possibility of controlling undesired side hydrolysis by enzyme engineering, have made glycosidases competitive synthetic tools. Current promising applications of engineered glycosidases include the production of well-defined chitooligomers, precious galactooligosaccharides or specialty chemicals such as glycosylated flavonoids. Other synthetic pathways leading to human milk oligosaccharides or remodeled antibodies are on the horizon. This work provides an overview of the synthetic achievements to date for glycosidases, emphasizing the latest trends and outlining possible developments in the field.

直到最近，天然水解碳水化合物活性酶的糖苷酶在工业中几乎没有发现合成应用，主要用于裂解不需要的碳水化合物。随着基因工程糖合酶和转糖苷酶技术的建立，糖苷酶作为工业生物技术工具的观点开始发生变化。它们易于生产、经济实惠、稳健性和底物多功能性，增加了通过酶工程控制不需要的侧水解的可能性，使糖苷酶成为具有竞争力的合成工具。目前工程糖苷酶的有前景的应用包括生产明确定义的壳寡糖、珍贵的低聚半乳糖或特殊化学品，如糖基化黄酮类化合物。导致人乳寡糖或重构抗体的其他合成途径即将出现。这项工作概述了迄今为止糖苷酶的合成成就，强调了最新趋势并概述了该领域的可能发展。