Hydrogels offer a promising potential for the encapsulation and regulated release of drugs due to their biocompatibility and their tunable properties as materials. Only a limited number of systems and procedures enable the encapsulation of sensitive proteins. N-terminally fmoc-protected phenylalanine has been shown to self-assemble into a transparent, stable hydrogel It can be considered a supergelator due to the low amount of monomers necessary for hydrogelation (0.1% w/v), making it a good candidate for the encapsulation and stabilization of sensitive proteins. However, application options for this hydrogel are rather limited to those of many other fibril-based materials due to its intrinsic lack of mechanical strength and high susceptibility to changes in environmental conditions. Here, we demonstrate that the stability of a fibrillary system and the resulting release of the protein-photosensitizer Azulitox can be increased by combining the hydrogel with a tightly cross-linked BSA hydrogel. Azulitox is known to display cell-penetrating properties, anti-proliferative activity and has a distinctive fluorescence. Confocal microscopy and fluorescence measurements verified the maintenance of all essential functions of the encapsulated protein. In contrast, the combination of fibrillary and protein hydrogel resulted in a significant stabilization of the matrix and an adjustable release pattern for encapsulated protein.

由于其生物相容性和作为材料的可调特性，水凝胶为药物的封装和调节释放提供了有希望的潜力。只有有限数量的系统和程序能够封装敏感蛋白质。N-末端 fmoc 保护的苯丙氨酸已被证明可以自组装成透明、稳定的水凝胶 由于水凝胶化所需的单体数量少（0.1% w/v），它可以被认为是一种超级凝胶剂，使其成为一个很好的候选者敏感蛋白质的封装和稳定。然而，由于其固有的机械强度不足和对环境条件变化的高度敏感性，这种水凝胶的应用选择相当受限于许多其他基于原纤维的材料。这里，我们证明了纤维系统的稳定性和由此产生的蛋白质光敏剂 Azulitox 的释放可以通过将水凝胶与紧密交联的 BSA 水凝胶结合来增加。众所周知，Azulitox 具有细胞穿透特性、抗增殖活性并具有独特的荧光。共聚焦显微镜和荧光测量验证了封装蛋白质的所有基本功能的维持。相比之下，原纤维和蛋白质水凝胶的组合导致基质的显着稳定性和封装蛋白质的可调节释放模式。具有抗增殖活性并具有独特的荧光。共聚焦显微镜和荧光测量验证了封装蛋白质的所有基本功能的维持。相比之下，原纤维和蛋白质水凝胶的组合导致基质的显着稳定性和封装蛋白质的可调节释放模式。具有抗增殖活性并具有独特的荧光。共聚焦显微镜和荧光测量验证了封装蛋白质的所有基本功能的维持。相比之下，原纤维和蛋白质水凝胶的组合导致基质的显着稳定性和封装蛋白质的可调节释放模式。