Abstract

Microalgal carbohydrate is considered one of the potential feedstock for biofuel produced via the bioconversion process. However, the current cultivation process using commercial medium exhibited low biomass production and its carbohydrate productivity which become a bottleneck for sustainable microalgal-carbohydrate-based biofuel production. Thus, the objective of this study is to assess the utilization of industrial waste including molasses and glycerol on the Halochlorella rubescens and Tetraselmis suecica growth as well as its carbohydrate content under different cultivation modes such as autotrophic, heterotrophic and photoheterotrophic conditions. From this study, the highest maximum biomass of H. rubenscens and T. suecica of 0.653 ± 0.009 and 0.669 ± 0.01gL⁻¹ were obtained when the cultivation was performed under photoheterotrophic using molasses. High carbohydrate content of H. rubescens and T. seucica of 56.81 ± 0.39% and 71.52 ± 0.03% with glucose represent the dominant sugar was observed under this condition. The growth kinetic model of the analysis indicated that Huang and Gompertz Models described well the growth of H. rubescens and T. suecica under photoheteroptroph condition with a high significant R² of 0.99. The information generated could be beneficial for the future development of low-cost microalgal cultivation media formulation for future microalgal carbohydrate-based products such as bioethanol.

摘要

微藻碳水化合物被认为是通过生物转化过程生产的生物燃料的潜在原料之一。然而，目前使用商业培养基的培养过程表现出低的生物质产量及其碳水化合物生产率，这成为可持续的基于微藻碳水化合物的生物燃料生产的瓶颈。因此，本研究的目的是评估工业废物（包括糖蜜和甘油）对Halochlorella rubescens和Tetraselmis suecica生长的利用及其在自养、异养和光异养等不同栽培模式下的碳水化合物含量。从这项研究中， H. rubenscens和T. suecica的最大最大生物量当使用糖蜜在光异养下进行培养时，分别获得了 0.653 ± 0.009 和 0.669 ± 0.01gL ^{-1 。}在此条件下观察到H. rubescens和T. seucica的高碳水化合物含量为56.81 ± 0.39% 和 71.52 ± 0.03%，其中葡萄糖是主要糖分。分析的生长动力学模型表明，Huang and Gompertz Models很好地描述了H. rubescens和T. suecica在光异养条件下的生长，具有高显着R ²0.99。所产生的信息可能有利于未来开发低成本的微藻培养基配方，用于未来基于微藻碳水化合物的产品，如生物乙醇。