PURPOSE Toxocara canis is a common intestinal nematode parasite of dogs with recognized zoonotic potential in tropical countries. The purpose of this study was to determine the seroprevalence of anti-T. canis antibodies in two target dog populations: household and community-owned, distributed over three distinct geographical regions of India. METHODS Two recombinant proteins of T. canis, cathepsin L-1 (CL-1) and Toxocara excretory-secretory-26 (TES-26), expressed in Escherichia coli, were used for studying the prevalence of anti-T. canis antibodies in dog populations in three distinct geographical regions of the country using an IgG-enzyme-linked immunosorbent assay. A total of 615 sera, 507 from household and 108 from community owned dogs were screened for IgG antibodies. RESULTS ELISA with recombinant (r) CL-1 showed 37.7% and 53.7% seroreactivity in household and community owned dogs, respectively. However, the rTES-26 antigen showed higher seroreactivity of 39.6% and 87.9% in the corresponding groups of household and community owned dogs, respectively. Chi-squared analysis of the data indicated that there was not any association in the prevalence of anti-T. canis antibodies between the samples analyzed from the three regions and the two cohorts of dog groups. However, the seroprevalence was higher in community owned dogs compared to household owned dogs. CONCLUSION The results of the serological evaluation suggest that both the groups of dogs show high seroreactivity rates and are likely to harbor T. canis infections of tissue dwelling dormant larvae.

中文翻译：

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在印度三个州用重组组织蛋白酶 L-1 和 TES-26 抗原检测犬抗弓形虫抗体的流行率。

目的 犬弓蛔虫是一种常见的犬肠道线虫寄生虫，在热带国家具有公认的人畜共患潜力。本研究的目的是确定抗 T 的血清阳性率。两种目标犬群中的犬抗体：家庭和社区拥有，分布在印度三个不同的地理区域。方法 使用在大肠杆菌中表达的犬T. canis 的两种重组蛋白组织蛋白酶L-1 (CL-1) 和Toxocara excretory-secretory-26 (TES-26) 来研究抗T 蛋白的流行情况。使用 IgG 酶联免疫吸附测定法在该国三个不同地理区域的狗群中检测犬类抗体。共筛选了 615 份血清、507 份来自家庭的血清和 108 份来自社区拥有的狗的 IgG 抗体。结果 ELISA 与重组 (r) CL-1 显示 37.7% 和 53。家庭和社区拥有的狗分别有 7% 的血清反应性。然而，rTES-26 抗原在相应的家庭和社区拥有的狗组中显示出更高的血清反应性，分别为 39.6% 和 87.9%。数据的卡方分析表明，抗 T 的流行没有任何关联。从三个区域和两组狗群分析的样本之间的犬抗体。然而，与家庭拥有的狗相比，社区拥有的狗的血清阳性率更高。结论 血清学评估结果表明，两组犬都表现出较高的血清反应率，并且很可能感染了组织寄居的休眠幼虫的犬 T. canis 感染。在相应的家庭和社区拥有的狗组中分别占 6% 和 87.9%。数据的卡方分析表明，抗 T 的流行没有任何关联。从三个区域和两组狗群分析的样本之间的犬抗体。然而，与家庭拥有的狗相比，社区拥有的狗的血清阳性率更高。结论 血清学评估结果表明，两组犬都表现出较高的血清反应率，并且很可能感染了组织寄居的休眠幼虫的犬 T. canis 感染。在相应的家庭和社区拥有的狗组中分别占 6% 和 87.9%。数据的卡方分析表明，抗 T 的流行没有任何关联。从三个区域和两组狗群分析的样本之间的犬抗体。然而，与家庭拥有的狗相比，社区拥有的狗的血清阳性率更高。结论 血清学评估结果表明，两组犬都表现出较高的血清反应率，并且很可能感染了组织寄居的休眠幼虫的犬 T. canis 感染。从三个区域和两组狗群分析的样本之间的犬抗体。然而，与家庭拥有的狗相比，社区拥有的狗的血清阳性率更高。结论 血清学评估结果表明，两组犬都表现出较高的血清反应率，并且很可能感染了组织寄居的休眠幼虫的犬 T. canis 感染。从三个区域和两组狗群分析的样本之间的犬抗体。然而，与家庭拥有的狗相比，社区拥有的狗的血清阳性率更高。结论 血清学评估结果表明，两组犬都表现出较高的血清反应率，并且很可能感染了组织寄居的休眠幼虫的犬 T. canis 感染。